Detecting seasonal variation of antifreeze protein distribution in Rhagium mordax using immunofluorescence and high resolution microscopy

Johannes Lørup Buch, Hans Ramløv

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

Larvae of the blackspotted pliers support beetle, Rhagium mordax, were collected monthly, for the duration of 2012 and fixed. The larvae were embedded in paraffin wax and sectioned. Using fluorophore-coupled antibodies specific to the R. mordax antifreeze protein, RmAFP1, sections were visualised with UV reflected light microscopy. An automated software analysis method was developed in order to discard autofluorescence, and quantify fluorescence from bound antibodies. The results show that R. mordax cuticle and gut exhibit a higher degree of fluorophore-bound fluorescence during summer, than in the cold months. It is hypothesised that R. mordax stores RmAFP1 in, or near, the fat body during times when freeze avoidance is not needed.
OriginalsprogEngelsk
TidsskriftCryobiology
Vol/bind74
Sider (fra-til)132-140
Antal sider9
ISSN0011-2240
DOI
StatusUdgivet - 2017

Bibliografisk note

The attached version of the article is wrongly specified as a 'Preprint'. This is the peer reviewed version.

Emneord

  • Antifreeze protein
  • Rhagium mordax
  • Tissue distribution
  • immunoflourescence

Citer dette