Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine

Tinkara Tinta, Louise Slot Christiansen, Anke Konrad, David A Liberles, Valentina Turk, Birgitte Munch-Petersen, Jure Piskur, Anders Ranegaard Clausen

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.
    OriginalsprogDansk
    TidsskriftF E M S Microbiology Letters
    Vol/bind331
    Udgave nummer2
    Sider (fra-til)120-127
    Antal sider8
    ISSN0378-1097
    DOI
    StatusUdgivet - jan. 2012

    Citer dette

    Tinta, Tinkara ; Christiansen, Louise Slot ; Konrad, Anke ; Liberles, David A ; Turk, Valentina ; Munch-Petersen, Birgitte ; Piskur, Jure ; Clausen, Anders Ranegaard. / Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine. I: F E M S Microbiology Letters. 2012 ; Bind 331, Nr. 2. s. 120-127.
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    abstract = "Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.",
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    Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine. / Tinta, Tinkara; Christiansen, Louise Slot; Konrad, Anke; Liberles, David A; Turk, Valentina; Munch-Petersen, Birgitte; Piskur, Jure; Clausen, Anders Ranegaard.

    I: F E M S Microbiology Letters, Bind 331, Nr. 2, 01.2012, s. 120-127.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

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    AU - Tinta, Tinkara

    AU - Christiansen, Louise Slot

    AU - Konrad, Anke

    AU - Liberles, David A

    AU - Turk, Valentina

    AU - Munch-Petersen, Birgitte

    AU - Piskur, Jure

    AU - Clausen, Anders Ranegaard

    PY - 2012/1

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