Danish Culicoides species of the Obsoletus group identified by morphological methods

Søren Achim Nielsen; M. Kristensen

Danish Culicoides species of the Obsoletus group identified by morphological methods

Søren Achim Nielsen, M. Kristensen

Miljødynamik

Publikation: Konferencebidrag › Konferenceabstrakt til konference › Forskning

Abstract

A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length.

PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%.

Conclusion: the importance of crossing different characters is highlighted to identify

species.

Originalsprog	Engelsk
Publikationsdato	2009
Antal sider	1
Status	Udgivet - 2009
Begivenhed	MedReoNet taxonomy meeting - Strasbourg, Frankrig Varighed: 16 mar. 2009 → 20 mar. 2009

Konference

Konference	MedReoNet taxonomy meeting
Land/Område	Frankrig
By	Strasbourg
Periode	16/03/2009 → 20/03/2009

Link til dokument

http://www.google.dk/url?sa=t&source=web&ct=res&cd=1&ved=0CAYQFjAA&url=http%3A%2F%2Fmedreonet.cirad.fr%2Fcontent%2Fdownload%2F1096%2F5651%2Ffile%2FMedReoNet_Minutes_VFinal.pdf&rct=j&q=avaritia+nielsen+strasbourg&ei=LCuOS-TVIsmQ-AbLyfTkCQ&usg=AFQjCNGVvrrjOVWzfWx9VurEPS7tM0F8sQ

Citer dette

Nielsen, S. A., & Kristensen, M. (2009). Danish Culicoides species of the Obsoletus group identified by morphological methods. Abstract fra MedReoNet taxonomy meeting, Strasbourg, Frankrig. http://www.google.dk/url?sa=t&source=web&ct=res&cd=1&ved=0CAYQFjAA&url=http%3A%2F%2Fmedreonet.cirad.fr%2Fcontent%2Fdownload%2F1096%2F5651%2Ffile%2FMedReoNet_Minutes_VFinal.pdf&rct=j&q=avaritia+nielsen+strasbourg&ei=LCuOS-TVIsmQ-AbLyfTkCQ&usg=AFQjCNGVvrrjOVWzfWx9VurEPS7tM0F8sQ

@conference{4e60872026b111df9df3000ea68e967b,

title = "Danish Culicoides species of the Obsoletus group identified by morphological methods",

abstract = "A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.",

author = "Nielsen, {S{\o}ren Achim} and M. Kristensen",

year = "2009",

language = "English",

note = "MedReoNet taxonomy meeting ; Conference date: 16-03-2009 Through 20-03-2009",

}

Nielsen, SA & Kristensen, M 2009, 'Danish Culicoides species of the Obsoletus group identified by morphological methods', MedReoNet taxonomy meeting, Strasbourg, Frankrig, 16/03/2009 - 20/03/2009. <http://www.google.dk/url?sa=t&source=web&ct=res&cd=1&ved=0CAYQFjAA&url=http%3A%2F%2Fmedreonet.cirad.fr%2Fcontent%2Fdownload%2F1096%2F5651%2Ffile%2FMedReoNet_Minutes_VFinal.pdf&rct=j&q=avaritia+nielsen+strasbourg&ei=LCuOS-TVIsmQ-AbLyfTkCQ&usg=AFQjCNGVvrrjOVWzfWx9VurEPS7tM0F8sQ>

TY - ABST

T1 - Danish Culicoides species of the Obsoletus group identified by morphological methods

AU - Nielsen, Søren Achim

AU - Kristensen, M.

PY - 2009

Y1 - 2009

N2 - A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.

AB - A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.

M3 - Conference abstract for conference

T2 - MedReoNet taxonomy meeting

Y2 - 16 March 2009 through 20 March 2009

ER -