Danish Culicoides species of the Obsoletus group identified by morphological methods

Søren Achim Nielsen, M. Kristensen

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskning

Resumé

A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length.

PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%.

Conclusion: the importance of crossing different characters is highlighted to identify

species.

OriginalsprogEngelsk
Publikationsdato2009
Antal sider1
StatusUdgivet - 2009
BegivenhedMedReoNet taxonomy meeting - Strasbourg, Frankrig
Varighed: 16 mar. 200920 mar. 2009

Konference

KonferenceMedReoNet taxonomy meeting
LandFrankrig
ByStrasbourg
Periode16/03/200920/03/2009

Citer dette

Nielsen, S. A., & Kristensen, M. (2009). Danish Culicoides species of the Obsoletus group identified by morphological methods. Abstract fra MedReoNet taxonomy meeting, Strasbourg, Frankrig.
Nielsen, Søren Achim ; Kristensen, M. / Danish Culicoides species of the Obsoletus group identified by morphological methods. Abstract fra MedReoNet taxonomy meeting, Strasbourg, Frankrig.1 s.
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abstract = "A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100{\%} agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1{\%} in C. obsoletus and about 3{\%} in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7{\%}. and about 3{\%} in ), when differences between and were about 5 to 7{\%}. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.",
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Nielsen, SA & Kristensen, M 2009, 'Danish Culicoides species of the Obsoletus group identified by morphological methods' MedReoNet taxonomy meeting, Strasbourg, Frankrig, 16/03/2009 - 20/03/2009, .

Danish Culicoides species of the Obsoletus group identified by morphological methods. / Nielsen, Søren Achim; Kristensen, M.

2009. Abstract fra MedReoNet taxonomy meeting, Strasbourg, Frankrig.

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskning

TY - ABST

T1 - Danish Culicoides species of the Obsoletus group identified by morphological methods

AU - Nielsen, Søren Achim

AU - Kristensen, M.

PY - 2009

Y1 - 2009

N2 - A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.

AB - A method was presented to morphologically separate C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus. From field-collected individuals, hypopygium and wing for male and head, wing, and spermatheca for female were observed and species identification was obtained using DNA assay. Differences between C. obsoletus and C. scoticus were found using the ratio of the length and width of the third segment of the palpus, the antennal ratio (sum of the lengths of the apical five segments of the the flagellum (11-15) to the sum of basal eight) and the wing length. Finally, there were few overlaps crossing the palpus ratio and the wing length. PCR identification showed a 100% agreement between morphology and molecular identification. ITS1 sequences were slightly variable within a species (less than 1% in C. obsoletus and about 3% in C. scoticus), when differences between C. obsoletus and C scoticus were about 5 to 7%. and about 3% in ), when differences between and were about 5 to 7%. Conclusion: the importance of crossing different characters is highlighted to identifyspecies.

M3 - Conference abstract for conference

ER -

Nielsen SA, Kristensen M. Danish Culicoides species of the Obsoletus group identified by morphological methods. 2009. Abstract fra MedReoNet taxonomy meeting, Strasbourg, Frankrig.