CPP1 a DNA-binding protein involved in the expression of a soybean leghemoglobin c3 gene

Cristina Cvitanich, Niels Pallisgaard, Kirsten A. Nielsen, Anette Chemnitz Hansen, Knud Larsen, Kaarina Pihakaski-Maunsbach, Kjeld A. Marcker, Erik Østergaard Jensen*

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Abstract

Nodulin genes are specifically expressed in the nitrogen-fixing root nodules. We have identified a novel type of DNA-binding protein (CPP1) interacting with the promoter of the soybean leghemoglobin gene Gmlbc3. The DNA-binding domain of CPP1 contains two similar Cys-rich domains with 9 and 10 Cys, respectively. Genes encoding similar domains have been identified in Arabidopsis thaliana, Caenorhabditis elegans, the mouse, and human. The domains also have some homology to a Cys-rich region present in some polycomb proteins. The cppl gene is induced late in nodule development and the expression is confined to the distal part of the central infected tissue of the nodule. A constitutively expressed cpp1 gene reduces the expression of a Gmlbc3 promoter-gusA reporter construct in Vicia hirsuta roots. These data therefore suggest that CPP1 might be involved in the regulation of the leghemoglobin genes in the symbiotic root nodule.

OriginalsprogEngelsk
TidsskriftProceedings of the National Academy of Sciences of the United States of America
Vol/bind97
Udgave nummer14
Sider (fra-til)8163-8168
Antal sider6
ISSN0027-8424
DOI
StatusUdgivet - 5 jul. 2000
Udgivet eksterntJa

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