Controls to validate plasma samples for cell free DNA quantification

Niels Pallisgaard*, Karen Lise Garm Spindler, Rikke Fredslund Andersen, Ivan Brandslund, Anders Jakobsen

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Abstract

Recent research has focused on the utility of cell free DNA (cfDNA) in serum and plasma for clinical application, especially in oncology. The literature holds promise of cfDNA as a valuable tumour marker to be used for treatment selection, monitoring and follow-up. The results, however, are diverging due to methodological differences with lack of standardisation and definition of sensitivity. The new biological information has not yet come into routine use.The present study presents external standardisation by spiking with non-human DNA fragments to control for loss of DNA during sample preparation and measurement. It also suggests a method to control for admixture of DNA from normal lymphocytes by utilizing the unique immunoglobulin gene rearrangement in the B-cells. The results show that this approach improves the quality of the analysis and lowers the risk of falsely increased values. In conclusion we suggest a new method to improve the accuracy of cfDNA measurements easily incorporated in the current technology.

OriginalsprogEngelsk
TidsskriftClinica Chimica Acta
Vol/bind446
Sider (fra-til)141-146
Antal sider6
ISSN0009-8981
DOI
StatusUdgivet - 5 jun. 2015
Udgivet eksterntJa

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