Comparison of methods for recovery of Ascaris suum larvae from tissues of mice

H. C. Slotved*, Lis Eriksen, K. Darwin Murrell, Peter Nansen

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Abstract

Experiments were undertaken to compare procedures for isolating Ascaris suum from mice. A standardised intragastric procedure for inoculation of A. suum eggs, which had a very low interdose variation (S.D.=6.2% of mean inoculation dose), was developed. There was no significant difference in the number of larvae recovered from the large intestine 4 h post inoculation (p.i.) by using either Baermannisation of washed intestinal wall or submucosal scrapings. In contrast, a significantly higher recovery of larvae was achieved by incubating the intestines vertically in a cylindrical saline- filled funnel. An agar-gel method was used to recover larvae from mucosal scrapings; however, the number of larvae recovered was the lowest of the methods tried. It was found that a significant increase in larval recovery from the liver at 24h p.i. resulted when the liver was pressed through a garlic press, rather than by using homogenisation. For lung recovery, the highest recovery of larvae (at day 8 p.i.) was obtained by disintegration of tissue with a pair of scissors and incorporating the tissue into agar-gel. The methods presented in this study may be useful for investigation of the histology, morphology and molecular biology of the early stages of A. suum.

OriginalsprogEngelsk
TidsskriftInternational Journal for Parasitology
Vol/bind27
Udgave nummer11
Sider (fra-til)1305-1310
Antal sider6
ISSN0020-7519
DOI
StatusUdgivet - nov. 1997
Udgivet eksterntJa

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