Characterization of the functional and anatomical differences in the atrial and ventricular myocardium from three species of elasmobranch fishes

smooth dogfish (Mustelus canis), sandbar shark (Carcharhinus plumbers), and clearnose skate (Raja eglanteria)

Julie Larsen, Peter Bushnell, John Steffensen, Morten Foldager Pedersen, Klaus Qvortrup, Richard Brill

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

We assessed the functional properties in atrial and ventricular myocardium (using isolated cardiac strips) of smooth dogfish (Mustelus canis), clearnose skate (Raja
eglanteria), and sandbar shark (Carcharhinus plumbeus) by blocking Ca2+ release from the sarcoplasmic reticulum (SR) with ryanodine and thapsigargin and measuring
the resultant changes in contraction–relaxation parameters and the force–frequency relationship at 20 °C and 30 °C. We also examined ultrastructural differences with electron microscopy. In tissues from smooth dogfish, net force (per cross-sectional area) and measures of the speeds of contraction and relaxation were all higher in atrial than ventricular myocardium at both temperatures. Atrial-ventricular differences were evident in the other two species primarily in measures of the rates of contraction and relaxation. Ryanodine-thapsigargin treatment reduced net force and its maximum positive first derivative (i.e., contractility), and increased time to 50 % relaxation in atrial tissue from smooth dogfish at 30 °C. It also increased times to peak force and half relaxation in clearnose skate atrial and ventricular tissue at both temperatures, but only in atrial tissue from sandbar shark at 30 °C; indicating that SR involvement
in excitation–contraction (EC) coupling is speciesand temperature-specific in elasmobranch fishes, as it is in teleost fishes. Atrial and ventricular myocardium from all three species displayed a negative force–frequency relationship, but there was no evidence that SR involvement in EC coupling was influenced by heart rate. SR was evident in electron micrographs, generally located in proximity to mitochondria and intercalated discs, and to a lesser extent between the myofibrils; with mitochondria being more numerous in ventricular than atrial myocardium in all three species
OriginalsprogEngelsk
TidsskriftJournal of Comparative Physiology B
Vol/bind187
Udgave nummer2
Sider (fra-til)291-313
Antal sider23
ISSN0174-1578
DOI
StatusUdgivet - feb. 2017

Citer dette

@article{0a9c2fdb0ff04de286549ea3f117b7e1,
title = "Characterization of the functional and anatomical differences in the atrial and ventricular myocardium from three species of elasmobranch fishes: smooth dogfish (Mustelus canis), sandbar shark (Carcharhinus plumbers), and clearnose skate (Raja eglanteria)",
abstract = "We assessed the functional properties in atrial and ventricular myocardium (using isolated cardiac strips) of smooth dogfish (Mustelus canis), clearnose skate (Raja eglanteria), and sandbar shark (Carcharhinus plumbeus) by blocking Ca2+ release from the sarcoplasmic reticulum (SR) with ryanodine and thapsigargin and measuring the resultant changes in contraction–relaxation parameters and the force–frequency relationship at 20 °C and 30 °C. We also examined ultrastructural differences with electron microscopy. In tissues from smooth dogfish, net force (per cross-sectional area) and measures of the speeds of contraction and relaxation were all higher in atrial than ventricular myocardium at both temperatures. Atrial-ventricular differences were evident in the other two species primarily in measures of the rates of contraction and relaxation. Ryanodine-thapsigargin treatment reduced net force and its maximum positive first derivative (i.e., contractility), and increased time to 50 {\%} relaxation in atrial tissue from smooth dogfish at 30 °C. It also increased times to peak force and half relaxation in clearnose skate atrial and ventricular tissue at both temperatures, but only in atrial tissue from sandbar shark at 30 °C; indicating that SR involvement in excitation–contraction (EC) coupling is species- and temperature-specific in elasmobranch fishes, as it is in teleost fishes. Atrial and ventricular myocardium from all three species displayed a negative force–frequency relationship, but there was no evidence that SR involvement in EC coupling was influenced by heart rate. SR was evident in electron micrographs, generally located in proximity to mitochondria and intercalated discs, and to a lesser extent between the myofibrils; with mitochondria being more numerous in ventricular than atrial myocardium in all three species",
author = "Julie Larsen and Peter Bushnell and John Steffensen and Pedersen, {Morten Foldager} and Klaus Qvortrup and Richard Brill",
year = "2017",
month = "2",
doi = "10.1007/s00360-016-1034-9",
language = "English",
volume = "187",
pages = "291--313",
journal = "Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology",
issn = "0174-1578",
publisher = "Physica-Verlag",
number = "2",

}

Characterization of the functional and anatomical differences in the atrial and ventricular myocardium from three species of elasmobranch fishes : smooth dogfish (Mustelus canis), sandbar shark (Carcharhinus plumbers), and clearnose skate (Raja eglanteria). / Larsen, Julie; Bushnell, Peter; Steffensen, John; Pedersen, Morten Foldager; Qvortrup, Klaus; Brill, Richard.

I: Journal of Comparative Physiology B, Bind 187, Nr. 2, 02.2017, s. 291-313.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Characterization of the functional and anatomical differences in the atrial and ventricular myocardium from three species of elasmobranch fishes

T2 - smooth dogfish (Mustelus canis), sandbar shark (Carcharhinus plumbers), and clearnose skate (Raja eglanteria)

AU - Larsen, Julie

AU - Bushnell, Peter

AU - Steffensen, John

AU - Pedersen, Morten Foldager

AU - Qvortrup, Klaus

AU - Brill, Richard

PY - 2017/2

Y1 - 2017/2

N2 - We assessed the functional properties in atrial and ventricular myocardium (using isolated cardiac strips) of smooth dogfish (Mustelus canis), clearnose skate (Raja eglanteria), and sandbar shark (Carcharhinus plumbeus) by blocking Ca2+ release from the sarcoplasmic reticulum (SR) with ryanodine and thapsigargin and measuring the resultant changes in contraction–relaxation parameters and the force–frequency relationship at 20 °C and 30 °C. We also examined ultrastructural differences with electron microscopy. In tissues from smooth dogfish, net force (per cross-sectional area) and measures of the speeds of contraction and relaxation were all higher in atrial than ventricular myocardium at both temperatures. Atrial-ventricular differences were evident in the other two species primarily in measures of the rates of contraction and relaxation. Ryanodine-thapsigargin treatment reduced net force and its maximum positive first derivative (i.e., contractility), and increased time to 50 % relaxation in atrial tissue from smooth dogfish at 30 °C. It also increased times to peak force and half relaxation in clearnose skate atrial and ventricular tissue at both temperatures, but only in atrial tissue from sandbar shark at 30 °C; indicating that SR involvement in excitation–contraction (EC) coupling is species- and temperature-specific in elasmobranch fishes, as it is in teleost fishes. Atrial and ventricular myocardium from all three species displayed a negative force–frequency relationship, but there was no evidence that SR involvement in EC coupling was influenced by heart rate. SR was evident in electron micrographs, generally located in proximity to mitochondria and intercalated discs, and to a lesser extent between the myofibrils; with mitochondria being more numerous in ventricular than atrial myocardium in all three species

AB - We assessed the functional properties in atrial and ventricular myocardium (using isolated cardiac strips) of smooth dogfish (Mustelus canis), clearnose skate (Raja eglanteria), and sandbar shark (Carcharhinus plumbeus) by blocking Ca2+ release from the sarcoplasmic reticulum (SR) with ryanodine and thapsigargin and measuring the resultant changes in contraction–relaxation parameters and the force–frequency relationship at 20 °C and 30 °C. We also examined ultrastructural differences with electron microscopy. In tissues from smooth dogfish, net force (per cross-sectional area) and measures of the speeds of contraction and relaxation were all higher in atrial than ventricular myocardium at both temperatures. Atrial-ventricular differences were evident in the other two species primarily in measures of the rates of contraction and relaxation. Ryanodine-thapsigargin treatment reduced net force and its maximum positive first derivative (i.e., contractility), and increased time to 50 % relaxation in atrial tissue from smooth dogfish at 30 °C. It also increased times to peak force and half relaxation in clearnose skate atrial and ventricular tissue at both temperatures, but only in atrial tissue from sandbar shark at 30 °C; indicating that SR involvement in excitation–contraction (EC) coupling is species- and temperature-specific in elasmobranch fishes, as it is in teleost fishes. Atrial and ventricular myocardium from all three species displayed a negative force–frequency relationship, but there was no evidence that SR involvement in EC coupling was influenced by heart rate. SR was evident in electron micrographs, generally located in proximity to mitochondria and intercalated discs, and to a lesser extent between the myofibrils; with mitochondria being more numerous in ventricular than atrial myocardium in all three species

U2 - 10.1007/s00360-016-1034-9

DO - 10.1007/s00360-016-1034-9

M3 - Journal article

VL - 187

SP - 291

EP - 313

JO - Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology

SN - 0174-1578

IS - 2

ER -