Identification of pathways involved in wound healing is important for understanding the pathogenesis of various intestinal diseases. Cellular inhibitor of apoptosis protein 2 (cIAP2) regulates proliferation and migration in non-epithelial cells and is expressed in human colonocytes. The aim of the study was to investigate the role of cIAP2 for wound healing in the normal human colon. Wound tissue was generated by taking rectosigmoidal biopsies across an experimental ulcer in healthy subjects after 5, 24 and 48 hours. In experimental ulcers, the expression of cIAP2 in regenerating intestinal epithelial cells (IECs) was increased at the wound edge after 24 hours (P < 0.05) and returned to normal after re-epithelialization and correlated with the inflammatory reaction in the experimental wounds (P < 0.001). cIAP2 was induced in vitro in regenerating Caco2 IECs after wound infliction (P < 0.01). Knock down of cIAP2 caused a substantial impairment of the IEC regeneration through inhibition of migration (P < 0.005). cIAP2 over-expression lead to formation of migrating IECs and upregulation of expression of RhoA and Rac1 as well as GTP-activation of Rac1. Transforming growth factor β1 enhanced the expression of cIAP2, but was not upregulated in wounds in vivo and in vitro. NF-κB and MAPK pathways did not affect cIAP2 expression. cIAP2 is in conclusion a regulator of human intestinal wound healing through enhanced migration along with activation of Rac1 and the findings suggest that cIAP2 could be a future therapeutic target to improve intestinal wound healing.
|Tidsskrift||American Journal of Physiology: Gastrointestinal and Liver Physiology|
|Status||Udgivet - 2015|
Seidelin, JB., Larsen, S., Linnemann, D., Vainer, B., Coskun, M., Troelsen, J. T., & Hagen Nielsen, O. (2015). Cellular inhibitor of apoptosis protein 2 (cIAP2) controls human colonic epithelial restitution, migration and Rac1 activation. American Journal of Physiology: Gastrointestinal and Liver Physiology, 308(2). https://doi.org/10.1152/ajpgi.00089.2014