Bioaccumulation, toxicokinetics, and effects of copper from sediment spiked with aqueous Cu, nano-CuO, or micro-CuO in the deposit-feeding snail, Potamopyrgus antipodarum

Chengfang Pang, Henriette Selck, Gary Thomas Banta, SUPERB K. MISRA, Deborah BERHANU, AGNIESZKA DYBOWSKA, Eugenia Valsami-Jones, Valery E. Forbes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The present study examined the relative importance of copper (aqueous Cu and CuO particles of different sizes) added to
sediment to determine the bioaccumulation, toxicokinetics, and effects in the deposit feeder Potamopyrgus antipodarum. In experiment 1,
the bioaccumulation of Cu (240 mg Cu/g dry wt of sediment) added as aqueous Cu (CuCl2), nano- (6 nm, 100 nm), or micro- (<5 mm)
CuO particles in adult snails was measured. In experiment 2, a more comprehensive analysis of the toxicokinetics of Cu (aqueous Cu,
6 nm, or 100 nm) was conducted. In experiment 3, the effects of Cu form (aqueous Cu and 6 nm CuO) on juvenile growth and survival at
0, 30, 60, 120, and 240 mg Cu/g dry weight sediment were assessed. Snails took up less of the 5-mm CuO particles than nano-CuO or
aqueous Cu. A substantial fraction of Cu taken up was associated with shell, and this was rapidly lost when snails were transferred to clean
sediment. Net uptake rates from sediment amended with 6 nm CuO and aqueous Cu were significantly higher (40–50%) than from
sediment amended with 100 nm CuO. During 2 wk of depuration, there were no significant differences in depuration rates (kd) among
forms (aqueous Cu: kd ¼ 0.12 wk1; 6 nm CuO: kd ¼ 0.22 wk1; 100 nm CuO: kd ¼ 0.2 wk1). Average juvenile growth was
reducedby 0.11 mm(41%) atmeasuredexposure concentrations of 127.2 mgCu/g dryweight sediment for aqueousCu and 71.9 mg Cu/g dry
weight sediment for 6 nm CuO compared with control; however, differences between forms were not statistically significant. Juvenile
snails in the highest exposure concentrations (aqueous Cu and 6-nm CuO groups pooled) reduced their growth by 0.18 mm on average
(67%) compared with the control group. Although we observed minor differences in toxicity among Cu forms, effects on juvenile snail
growth occurred at bulk sediment concentrations lower than those in the Canadian interim sediment quality guidelines. Characterization of
the CuO particles showed that particle size distributions of commercially prepared particles deviated substantially from the manufacturers’
specifications and highlighted the importance of fully characterizing particles when using them in toxicity tests.
OriginalsprogEngelsk
TidsskriftEnvironmental Toxicology and Chemistry
Vol/bind32
Udgave nummer7
Sider (fra-til)1561–1573
ISSN0730-7268
DOI
StatusUdgivet - 2013

Citer dette

Pang, Chengfang ; Selck, Henriette ; Banta, Gary Thomas ; K. MISRA, SUPERB ; BERHANU, Deborah ; DYBOWSKA, AGNIESZKA ; Valsami-Jones, Eugenia ; Forbes, Valery E. / Bioaccumulation, toxicokinetics, and effects of copper from sediment spiked with aqueous Cu, nano-CuO, or micro-CuO in the deposit-feeding snail, Potamopyrgus antipodarum. I: Environmental Toxicology and Chemistry. 2013 ; Bind 32, Nr. 7. s. 1561–1573.
@article{99ebfb162dd24c6895042d64e32fc5c5,
title = "Bioaccumulation, toxicokinetics, and effects of copper from sediment spiked with aqueous Cu, nano-CuO, or micro-CuO in the deposit-feeding snail, Potamopyrgus antipodarum",
abstract = "The present study examined the relative importance of copper (aqueous Cu and CuO particles of different sizes) added tosediment to determine the bioaccumulation, toxicokinetics, and effects in the deposit feeder Potamopyrgus antipodarum. In experiment 1,the bioaccumulation of Cu (240 mg Cu/g dry wt of sediment) added as aqueous Cu (CuCl2), nano- (6 nm, 100 nm), or micro- (<5 mm)CuO particles in adult snails was measured. In experiment 2, a more comprehensive analysis of the toxicokinetics of Cu (aqueous Cu,6 nm, or 100 nm) was conducted. In experiment 3, the effects of Cu form (aqueous Cu and 6 nm CuO) on juvenile growth and survival at0, 30, 60, 120, and 240 mg Cu/g dry weight sediment were assessed. Snails took up less of the 5-mm CuO particles than nano-CuO oraqueous Cu. A substantial fraction of Cu taken up was associated with shell, and this was rapidly lost when snails were transferred to cleansediment. Net uptake rates from sediment amended with 6 nm CuO and aqueous Cu were significantly higher (40–50{\%}) than fromsediment amended with 100 nm CuO. During 2 wk of depuration, there were no significant differences in depuration rates (kd) amongforms (aqueous Cu: kd ¼ 0.12 wk1; 6 nm CuO: kd ¼ 0.22 wk1; 100 nm CuO: kd ¼ 0.2 wk1). Average juvenile growth wasreducedby 0.11 mm(41{\%}) atmeasuredexposure concentrations of 127.2 mgCu/g dryweight sediment for aqueousCu and 71.9 mg Cu/g dryweight sediment for 6 nm CuO compared with control; however, differences between forms were not statistically significant. Juvenilesnails in the highest exposure concentrations (aqueous Cu and 6-nm CuO groups pooled) reduced their growth by 0.18 mm on average(67{\%}) compared with the control group. Although we observed minor differences in toxicity among Cu forms, effects on juvenile snailgrowth occurred at bulk sediment concentrations lower than those in the Canadian interim sediment quality guidelines. Characterization ofthe CuO particles showed that particle size distributions of commercially prepared particles deviated substantially from the manufacturers’specifications and highlighted the importance of fully characterizing particles when using them in toxicity tests.",
author = "Chengfang Pang and Henriette Selck and Banta, {Gary Thomas} and {K. MISRA}, SUPERB and Deborah BERHANU and AGNIESZKA DYBOWSKA and Eugenia Valsami-Jones and Forbes, {Valery E.}",
year = "2013",
doi = "10.1002/etc.2216",
language = "English",
volume = "32",
pages = "1561–1573",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "JohnWiley & Sons, Inc.",
number = "7",

}

Bioaccumulation, toxicokinetics, and effects of copper from sediment spiked with aqueous Cu, nano-CuO, or micro-CuO in the deposit-feeding snail, Potamopyrgus antipodarum. / Pang, Chengfang; Selck, Henriette; Banta, Gary Thomas; K. MISRA, SUPERB; BERHANU, Deborah; DYBOWSKA, AGNIESZKA; Valsami-Jones, Eugenia; Forbes, Valery E.

I: Environmental Toxicology and Chemistry, Bind 32, Nr. 7, 2013, s. 1561–1573.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Bioaccumulation, toxicokinetics, and effects of copper from sediment spiked with aqueous Cu, nano-CuO, or micro-CuO in the deposit-feeding snail, Potamopyrgus antipodarum

AU - Pang, Chengfang

AU - Selck, Henriette

AU - Banta, Gary Thomas

AU - K. MISRA, SUPERB

AU - BERHANU, Deborah

AU - DYBOWSKA, AGNIESZKA

AU - Valsami-Jones, Eugenia

AU - Forbes, Valery E.

PY - 2013

Y1 - 2013

N2 - The present study examined the relative importance of copper (aqueous Cu and CuO particles of different sizes) added tosediment to determine the bioaccumulation, toxicokinetics, and effects in the deposit feeder Potamopyrgus antipodarum. In experiment 1,the bioaccumulation of Cu (240 mg Cu/g dry wt of sediment) added as aqueous Cu (CuCl2), nano- (6 nm, 100 nm), or micro- (<5 mm)CuO particles in adult snails was measured. In experiment 2, a more comprehensive analysis of the toxicokinetics of Cu (aqueous Cu,6 nm, or 100 nm) was conducted. In experiment 3, the effects of Cu form (aqueous Cu and 6 nm CuO) on juvenile growth and survival at0, 30, 60, 120, and 240 mg Cu/g dry weight sediment were assessed. Snails took up less of the 5-mm CuO particles than nano-CuO oraqueous Cu. A substantial fraction of Cu taken up was associated with shell, and this was rapidly lost when snails were transferred to cleansediment. Net uptake rates from sediment amended with 6 nm CuO and aqueous Cu were significantly higher (40–50%) than fromsediment amended with 100 nm CuO. During 2 wk of depuration, there were no significant differences in depuration rates (kd) amongforms (aqueous Cu: kd ¼ 0.12 wk1; 6 nm CuO: kd ¼ 0.22 wk1; 100 nm CuO: kd ¼ 0.2 wk1). Average juvenile growth wasreducedby 0.11 mm(41%) atmeasuredexposure concentrations of 127.2 mgCu/g dryweight sediment for aqueousCu and 71.9 mg Cu/g dryweight sediment for 6 nm CuO compared with control; however, differences between forms were not statistically significant. Juvenilesnails in the highest exposure concentrations (aqueous Cu and 6-nm CuO groups pooled) reduced their growth by 0.18 mm on average(67%) compared with the control group. Although we observed minor differences in toxicity among Cu forms, effects on juvenile snailgrowth occurred at bulk sediment concentrations lower than those in the Canadian interim sediment quality guidelines. Characterization ofthe CuO particles showed that particle size distributions of commercially prepared particles deviated substantially from the manufacturers’specifications and highlighted the importance of fully characterizing particles when using them in toxicity tests.

AB - The present study examined the relative importance of copper (aqueous Cu and CuO particles of different sizes) added tosediment to determine the bioaccumulation, toxicokinetics, and effects in the deposit feeder Potamopyrgus antipodarum. In experiment 1,the bioaccumulation of Cu (240 mg Cu/g dry wt of sediment) added as aqueous Cu (CuCl2), nano- (6 nm, 100 nm), or micro- (<5 mm)CuO particles in adult snails was measured. In experiment 2, a more comprehensive analysis of the toxicokinetics of Cu (aqueous Cu,6 nm, or 100 nm) was conducted. In experiment 3, the effects of Cu form (aqueous Cu and 6 nm CuO) on juvenile growth and survival at0, 30, 60, 120, and 240 mg Cu/g dry weight sediment were assessed. Snails took up less of the 5-mm CuO particles than nano-CuO oraqueous Cu. A substantial fraction of Cu taken up was associated with shell, and this was rapidly lost when snails were transferred to cleansediment. Net uptake rates from sediment amended with 6 nm CuO and aqueous Cu were significantly higher (40–50%) than fromsediment amended with 100 nm CuO. During 2 wk of depuration, there were no significant differences in depuration rates (kd) amongforms (aqueous Cu: kd ¼ 0.12 wk1; 6 nm CuO: kd ¼ 0.22 wk1; 100 nm CuO: kd ¼ 0.2 wk1). Average juvenile growth wasreducedby 0.11 mm(41%) atmeasuredexposure concentrations of 127.2 mgCu/g dryweight sediment for aqueousCu and 71.9 mg Cu/g dryweight sediment for 6 nm CuO compared with control; however, differences between forms were not statistically significant. Juvenilesnails in the highest exposure concentrations (aqueous Cu and 6-nm CuO groups pooled) reduced their growth by 0.18 mm on average(67%) compared with the control group. Although we observed minor differences in toxicity among Cu forms, effects on juvenile snailgrowth occurred at bulk sediment concentrations lower than those in the Canadian interim sediment quality guidelines. Characterization ofthe CuO particles showed that particle size distributions of commercially prepared particles deviated substantially from the manufacturers’specifications and highlighted the importance of fully characterizing particles when using them in toxicity tests.

U2 - 10.1002/etc.2216

DO - 10.1002/etc.2216

M3 - Journal article

VL - 32

SP - 1561

EP - 1573

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 7

ER -