Basal and T3-induced ROS Production in Lymphocyte Mitochondria is Increased in Type 2 Diabetic Patients

Stine Anthonsen, Jacob Larsen, Palle Lyngsie Pedersen, Louise Torp Dalgaard, Jan Kvetny

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Mitochondrial function, including production of reactive oxygen species (ROS), is important in the pathogenesis of diabetes and its complications. Thyroid hormones are major regulator of these processes. Hence, the aim of this study was to examine the thyroid hormone regulation of ROS production in human lymphocytes in patients with diabetes mellitus type 2 (T2DM). Lymphocytes from 10 controls and 10 persons with T2DM were examined. Mitochondrial membrane potential (MMP) was examined by flow cytometry after staining with MitoTracker Green (MTG). Similarly ROS was measured following staining with carboxy-H2DCFDA. MMP was increased in T2DM patients and T3 stimulation increased MMP in controls [1 398 a.u. (979-4 094) vs. 2 156 a.u. (1 611-15 189), p=0.04, median and quartiles] as well as in T2DM patients [9 167 a.u. (7 387-11 746) vs. 20 274 a.u. (17 183-27 839 p=0.004, median and quartiles]. Basal ROS concentration was increased in lymphocytes from T2DM and T3 significantly stimulated ROS concentration in controls [3 691 a.u. (2 584-6 396) vs. 5 650 a.u. (3 001-7 802) p=0.013, median and quartiles] and in T2DM patients [19 271 a.u. (6 288-25 282) vs. 23 178 a.u. (10 004-28 857) p=0.013, median and quartiles]. The ratio of ROS production related to MMP was significantly higher in T2DM, unstimulated as well as T3-stimulated in T2DM. Unstimulated and T3 stimulated ROS production and MMP were higher in lymphocytes from diabetic patients. An altered balance between ROS production and MMP, favoring ROS production in T2DM patients, was found suggesting that an increased mitochondrial sensitivity for T3 may be a significant factor responsible for increased ROS activity in diabetic patients.
    OriginalsprogEngelsk
    TidsskriftHormone and Metabolic Research
    Vol/bind45
    Udgave nummer4
    Sider (fra-til)261-266
    ISSN0018-5043
    DOI
    StatusUdgivet - 2013

    Emneord

    • diabetes mellitus type 2
    • thyroid hormone
    • mitochondria
    • ROS
    • MMP

    Citer dette

    Anthonsen, Stine ; Larsen, Jacob ; Pedersen, Palle Lyngsie ; Dalgaard, Louise Torp ; Kvetny, Jan. / Basal and T3-induced ROS Production in Lymphocyte Mitochondria is Increased in Type 2 Diabetic Patients. I: Hormone and Metabolic Research. 2013 ; Bind 45, Nr. 4. s. 261-266.
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    title = "Basal and T3-induced ROS Production in Lymphocyte Mitochondria is Increased in Type 2 Diabetic Patients",
    abstract = "Mitochondrial function, including production of reactive oxygen species (ROS), is important in the pathogenesis of diabetes and its complications. Thyroid hormones are major regulator of these processes. Hence, the aim of this study was to examine the thyroid hormone regulation of ROS production in human lymphocytes in patients with diabetes mellitus type 2 (T2DM). Lymphocytes from 10 controls and 10 persons with T2DM were examined. Mitochondrial membrane potential (MMP) was examined by flow cytometry after staining with MitoTracker Green (MTG). Similarly ROS was measured following staining with carboxy-H2DCFDA. MMP was increased in T2DM patients and T3 stimulation increased MMP in controls [1 398 a.u. (979-4 094) vs. 2 156 a.u. (1 611-15 189), p=0.04, median and quartiles] as well as in T2DM patients [9 167 a.u. (7 387-11 746) vs. 20 274 a.u. (17 183-27 839 p=0.004, median and quartiles]. Basal ROS concentration was increased in lymphocytes from T2DM and T3 significantly stimulated ROS concentration in controls [3 691 a.u. (2 584-6 396) vs. 5 650 a.u. (3 001-7 802) p=0.013, median and quartiles] and in T2DM patients [19 271 a.u. (6 288-25 282) vs. 23 178 a.u. (10 004-28 857) p=0.013, median and quartiles]. The ratio of ROS production related to MMP was significantly higher in T2DM, unstimulated as well as T3-stimulated in T2DM. Unstimulated and T3 stimulated ROS production and MMP were higher in lymphocytes from diabetic patients. An altered balance between ROS production and MMP, favoring ROS production in T2DM patients, was found suggesting that an increased mitochondrial sensitivity for T3 may be a significant factor responsible for increased ROS activity in diabetic patients.",
    keywords = "diabetes mellitus type 2, thyroid hormone , mitochondria , ROS , MMP",
    author = "Stine Anthonsen and Jacob Larsen and Pedersen, {Palle Lyngsie} and Dalgaard, {Louise Torp} and Jan Kvetny",
    year = "2013",
    doi = "10.1055/s-0032-1327590",
    language = "English",
    volume = "45",
    pages = "261--266",
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    Basal and T3-induced ROS Production in Lymphocyte Mitochondria is Increased in Type 2 Diabetic Patients. / Anthonsen, Stine; Larsen, Jacob; Pedersen, Palle Lyngsie; Dalgaard, Louise Torp; Kvetny, Jan.

    I: Hormone and Metabolic Research, Bind 45, Nr. 4, 2013, s. 261-266.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Basal and T3-induced ROS Production in Lymphocyte Mitochondria is Increased in Type 2 Diabetic Patients

    AU - Anthonsen, Stine

    AU - Larsen, Jacob

    AU - Pedersen, Palle Lyngsie

    AU - Dalgaard, Louise Torp

    AU - Kvetny, Jan

    PY - 2013

    Y1 - 2013

    N2 - Mitochondrial function, including production of reactive oxygen species (ROS), is important in the pathogenesis of diabetes and its complications. Thyroid hormones are major regulator of these processes. Hence, the aim of this study was to examine the thyroid hormone regulation of ROS production in human lymphocytes in patients with diabetes mellitus type 2 (T2DM). Lymphocytes from 10 controls and 10 persons with T2DM were examined. Mitochondrial membrane potential (MMP) was examined by flow cytometry after staining with MitoTracker Green (MTG). Similarly ROS was measured following staining with carboxy-H2DCFDA. MMP was increased in T2DM patients and T3 stimulation increased MMP in controls [1 398 a.u. (979-4 094) vs. 2 156 a.u. (1 611-15 189), p=0.04, median and quartiles] as well as in T2DM patients [9 167 a.u. (7 387-11 746) vs. 20 274 a.u. (17 183-27 839 p=0.004, median and quartiles]. Basal ROS concentration was increased in lymphocytes from T2DM and T3 significantly stimulated ROS concentration in controls [3 691 a.u. (2 584-6 396) vs. 5 650 a.u. (3 001-7 802) p=0.013, median and quartiles] and in T2DM patients [19 271 a.u. (6 288-25 282) vs. 23 178 a.u. (10 004-28 857) p=0.013, median and quartiles]. The ratio of ROS production related to MMP was significantly higher in T2DM, unstimulated as well as T3-stimulated in T2DM. Unstimulated and T3 stimulated ROS production and MMP were higher in lymphocytes from diabetic patients. An altered balance between ROS production and MMP, favoring ROS production in T2DM patients, was found suggesting that an increased mitochondrial sensitivity for T3 may be a significant factor responsible for increased ROS activity in diabetic patients.

    AB - Mitochondrial function, including production of reactive oxygen species (ROS), is important in the pathogenesis of diabetes and its complications. Thyroid hormones are major regulator of these processes. Hence, the aim of this study was to examine the thyroid hormone regulation of ROS production in human lymphocytes in patients with diabetes mellitus type 2 (T2DM). Lymphocytes from 10 controls and 10 persons with T2DM were examined. Mitochondrial membrane potential (MMP) was examined by flow cytometry after staining with MitoTracker Green (MTG). Similarly ROS was measured following staining with carboxy-H2DCFDA. MMP was increased in T2DM patients and T3 stimulation increased MMP in controls [1 398 a.u. (979-4 094) vs. 2 156 a.u. (1 611-15 189), p=0.04, median and quartiles] as well as in T2DM patients [9 167 a.u. (7 387-11 746) vs. 20 274 a.u. (17 183-27 839 p=0.004, median and quartiles]. Basal ROS concentration was increased in lymphocytes from T2DM and T3 significantly stimulated ROS concentration in controls [3 691 a.u. (2 584-6 396) vs. 5 650 a.u. (3 001-7 802) p=0.013, median and quartiles] and in T2DM patients [19 271 a.u. (6 288-25 282) vs. 23 178 a.u. (10 004-28 857) p=0.013, median and quartiles]. The ratio of ROS production related to MMP was significantly higher in T2DM, unstimulated as well as T3-stimulated in T2DM. Unstimulated and T3 stimulated ROS production and MMP were higher in lymphocytes from diabetic patients. An altered balance between ROS production and MMP, favoring ROS production in T2DM patients, was found suggesting that an increased mitochondrial sensitivity for T3 may be a significant factor responsible for increased ROS activity in diabetic patients.

    KW - diabetes mellitus type 2

    KW - thyroid hormone

    KW - mitochondria

    KW - ROS

    KW - MMP

    U2 - 10.1055/s-0032-1327590

    DO - 10.1055/s-0032-1327590

    M3 - Journal article

    VL - 45

    SP - 261

    EP - 266

    JO - Hormone and Metabolic Research

    JF - Hormone and Metabolic Research

    SN - 0018-5043

    IS - 4

    ER -