Resumé
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Methods in Molecular Biology |
| Vol/bind | 2029 |
| Sider (fra-til) | 25-35 |
| Antal sider | 11 |
| ISSN | 1064-3745 |
| DOI | |
| Status | Udgivet - 2019 |
Citer dette
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Assessment of β-Cell Replication in Isolated Rat Islets of Langerhans. / Dalgaard, Louise Torp.
I: Methods in Molecular Biology, Bind 2029, 2019, s. 25-35.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
TY - JOUR
T1 - Assessment of β-Cell Replication in Isolated Rat Islets of Langerhans
AU - Dalgaard, Louise Torp
PY - 2019
Y1 - 2019
N2 - Pancreatic β-cells in the islets of Langerhans secrete insulin in response to the rise in glucose levels following food intake. The hypoglycemic action of insulin applies a strong evolutionary brake on β-cell division. However, under some conditions β-cells can be stimulated to enter cell cycle progression and divide, for example following exposure to increased glucose levels or during pregnancy. Here, a protocol is described for the isolation of rat adult islets of Langerhans, followed by culture of intact islets in Matrigel and measurement of β-cell replication by the incorporation of ethynyldeoxyuridine (EdU). EdU positive cells are revealed by a click reaction, nuclei are visualized using a DNA-binding fluorophore (Hoechst 33342), and β-cells are identified using immunofluorescence detection.
AB - Pancreatic β-cells in the islets of Langerhans secrete insulin in response to the rise in glucose levels following food intake. The hypoglycemic action of insulin applies a strong evolutionary brake on β-cell division. However, under some conditions β-cells can be stimulated to enter cell cycle progression and divide, for example following exposure to increased glucose levels or during pregnancy. Here, a protocol is described for the isolation of rat adult islets of Langerhans, followed by culture of intact islets in Matrigel and measurement of β-cell replication by the incorporation of ethynyldeoxyuridine (EdU). EdU positive cells are revealed by a click reaction, nuclei are visualized using a DNA-binding fluorophore (Hoechst 33342), and β-cells are identified using immunofluorescence detection.
U2 - 10.1007/978-1-4939-9631-5_3
DO - 10.1007/978-1-4939-9631-5_3
M3 - Journal article
VL - 2029
SP - 25
EP - 35
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -