Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Clovis Macêdo Bezerra Filho*, Luis Cláudio Nascimento da Silva*, Márcia Vanusa da Silva, Anders Løbner-Olesen, Carsten Struve, Karen Angeliki Krogfelt, Maria Tereza dos Santos Correia, Maria Luiza Vilela Oliva*

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

Eugenia brejoensis L. (Myrtaceae) is an endemic plant from caatinga ecosystem
(brazilian semi-arid) which have an E. brejoensis essential oil (EbEO) with reported
antimicrobial activity. In this work, in vitro and in vivo models were used to characterize
the inhibitory effects of EbEO in relation to Staphylococcus aureus. EbEO inhibited
the growth of all tested S. aureus strains (including multidrug resistance isolates)
with values ranging from 8 to 516 µg/mL. EbEO also synergistically increased the
action of ampicillim, chloramphenicol, and kanamycin. The treatment with subinhibitory
concentrations (Sub-MIC) of EbEO decreased S. aureus hemolytic activity and its ability
to survive in human blood. EbEO strongly reduced the levels of staphyloxanthin (STX),
an effect related to increased susceptibility of S. aureus to hydrogen peroxide. The
efficacy of EbEO against S. aureus was further demonstrated using Caenorhabditis
elegans and Galleria mellonella. EbEO increased the lifespan of both organisms infected
by S. aureus, reducing the bacterial load. In addition, EbEO reduced the severity of
S. aureus infection in G. mellonella, as shown by lower levels of melanin production
in those larvae. In summary, our data suggest that EbEO is a potential source of lead
molecules for development of new therapeutic alternatives against S. aureus
OriginalsprogEngelsk
Artikelnummer424
TidsskriftFrontiers in Microbiology
Vol/bind2020
Udgave nummer11
ISSN1664-302X
DOI
StatusUdgivet - 2020

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