TY - JOUR
T1 - Anti-staphylococcal activity of Syagrus coronata essential oil
T2 - Biofilm eradication and in vivo action on Galleria mellonela infection model
AU - dos Santos, Bruno Souza
AU - Macedo Bezerra Filho, Clóvis
AU - Adelson Alves do Nascimento Junior, José
AU - Brust, Flávia Roberta
AU - Bezerra-Silva, Patrícia Cristina
AU - da Rocha, Suyana Karoline Lino
AU - Krogfelt, K. A.
AU - do Amaral Ferraz Navarro, Daniela Maria
AU - dos Santos Correia, Maria Tereza
AU - Napoleão, Thiago Henrique
AU - da Silva, Luís Claudio Nascimento
AU - Macedo, Alexandre José
AU - da Silva, Márcia Vanusa
AU - Guedes Paiva, Patrícia Maria
PY - 2019
Y1 - 2019
N2 - In this study, essential oil extracted from Syagrus coronata seeds (SCEO) was evaluated for antibacterial and antibiofilm activities against Staphylococcus aureus; in addition, Galleria mellonella model was used as an in vivo infection model. SCEO was mainly composed by fatty acids (89.79%) and sesquiterpenes (8.5%). The major components were octanoic acid, dodecanoic acid, decanoic acid and γ-eudesmol. SCEO showed bactericidal activity (minimal bactericidal concentration from 312 to 1250 μg/mL) against all tested S. aureus clinical isolates, which showed distinct biofilm-forming and multiple drug resistance phenotypes. SCEO weakly reduced biomass but remarkably decreased cell viability in pre-formed biofilms of S. aureus isolate UFPEDA-02 (ATCC-6538). Electron microscopy analysis showed that SCEO treatments decreased the number of bacterial cells (causing structural alterations) and lead to loss of the roughness in the multiple layers of the three-dimensional biofilm structure. In addition, overproduction of exopolymeric matrix was observed. SCEO at 31.2 mg/kg improved the survival of G. mellonela larvae inoculated with UFPEDA-02 isolate and reduced the bacterial load in hemolymph and melanization. In conclusion, SCEO is an antibacterial agent against S. aureus strains with different resistance phenotypes and able to disturb biofilm architecture. Our results show SCEO as a potential candidate to drug development.
AB - In this study, essential oil extracted from Syagrus coronata seeds (SCEO) was evaluated for antibacterial and antibiofilm activities against Staphylococcus aureus; in addition, Galleria mellonella model was used as an in vivo infection model. SCEO was mainly composed by fatty acids (89.79%) and sesquiterpenes (8.5%). The major components were octanoic acid, dodecanoic acid, decanoic acid and γ-eudesmol. SCEO showed bactericidal activity (minimal bactericidal concentration from 312 to 1250 μg/mL) against all tested S. aureus clinical isolates, which showed distinct biofilm-forming and multiple drug resistance phenotypes. SCEO weakly reduced biomass but remarkably decreased cell viability in pre-formed biofilms of S. aureus isolate UFPEDA-02 (ATCC-6538). Electron microscopy analysis showed that SCEO treatments decreased the number of bacterial cells (causing structural alterations) and lead to loss of the roughness in the multiple layers of the three-dimensional biofilm structure. In addition, overproduction of exopolymeric matrix was observed. SCEO at 31.2 mg/kg improved the survival of G. mellonela larvae inoculated with UFPEDA-02 isolate and reduced the bacterial load in hemolymph and melanization. In conclusion, SCEO is an antibacterial agent against S. aureus strains with different resistance phenotypes and able to disturb biofilm architecture. Our results show SCEO as a potential candidate to drug development.
KW - Antibacterial activity
KW - Antibiofilm
KW - Staphylococcus aureus
KW - Volatile oil
U2 - 10.1016/j.micpath.2019.04.009
DO - 10.1016/j.micpath.2019.04.009
M3 - Journal article
SN - 0882-4010
VL - 131
SP - 150
EP - 157
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
ER -