An open source cryostage and software analysis method for detection of antifreeze activity

Johannes Lørup Buch, Hans Ramløv

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

The aim of this study is to provide the reader with a simple setup that can detect antifreeze proteins (AFP) by inhibition of ice recrystallisation in very small sample sizes. This includes an open source cryostage, a method for preparing and loading samples as well as a software analysis method. The entire setup was tested using hyperactive AFP from the cerambycid beetle, Rhagium mordax. Samples containing AFP were compared to buffer samples, and the results are visualised as crystal radius evolution over time and in absolute change over 30 min. Statistical analysis showed that samples containing AFP could reliably be told apart from controls after only two minutes of recrystallisation. The goal of providing a fast, cheap and easy method for detecting antifreeze proteins in solution was met, and further development of the system can be followed at https://github.com/pechano/cryostage.
OriginalsprogEngelsk
TidsskriftCryobiology
Vol/bind72
Udgave nummer3
Sider (fra-til)251-257
ISSN0011-2240
DOI
StatusUdgivet - 31 mar. 2016

Emneord

  • cryostage antifreeze protein ice recrystallisation

Citer dette