An NMR-based metabolomic approach to assess metabolism in splanchnic tissues of steers

H.C. Bertram, N.B. Kristensen, A. Malmendal, N.C. Nielsen, S.K. Jensen, D.L. Harmon

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

1H NMR spectra were acquired on plasma samples obtained from the mesenteric artery, hepatic portal vein and the hepatic vein of steers (n = 6) to demonstrate a NMR-based metabolomic approach to study splanchnic metabolism. The metabolic differences between the different plasma samples (arterial, portal, hepatic) were examined by partial least square regressions (PLSR) on the obtained spectra. These analyses revealed spectral differences that could be ascribed to changes in the level of propionate, $-hydroxybutyrate, lactate, acetate, n-butyrate and glucose, which was supported by biochemical analyses.
OriginalsprogEngelsk
TidsskriftJournal of Animal and Feed Sciences
Vol/bind13
Udgave nummerSUPPL. 1
DOI
StatusUdgivet - 2004

Emneord

  • Metabolism
  • Metabolomics
  • NMR
  • Plasma
  • Splanchnic
  • Steer

Citer dette

Bertram, H.C. ; Kristensen, N.B. ; Malmendal, A. ; Nielsen, N.C. ; Jensen, S.K. ; Harmon, D.L. / An NMR-based metabolomic approach to assess metabolism in splanchnic tissues of steers. I: Journal of Animal and Feed Sciences. 2004 ; Bind 13, Nr. SUPPL. 1.
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An NMR-based metabolomic approach to assess metabolism in splanchnic tissues of steers. / Bertram, H.C.; Kristensen, N.B.; Malmendal, A.; Nielsen, N.C.; Jensen, S.K.; Harmon, D.L.

I: Journal of Animal and Feed Sciences, Bind 13, Nr. SUPPL. 1, 2004.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - An NMR-based metabolomic approach to assess metabolism in splanchnic tissues of steers

AU - Bertram, H.C.

AU - Kristensen, N.B.

AU - Malmendal, A.

AU - Nielsen, N.C.

AU - Jensen, S.K.

AU - Harmon, D.L.

PY - 2004

Y1 - 2004

N2 - 1H NMR spectra were acquired on plasma samples obtained from the mesenteric artery, hepatic portal vein and the hepatic vein of steers (n = 6) to demonstrate a NMR-based metabolomic approach to study splanchnic metabolism. The metabolic differences between the different plasma samples (arterial, portal, hepatic) were examined by partial least square regressions (PLSR) on the obtained spectra. These analyses revealed spectral differences that could be ascribed to changes in the level of propionate, $-hydroxybutyrate, lactate, acetate, n-butyrate and glucose, which was supported by biochemical analyses.

AB - 1H NMR spectra were acquired on plasma samples obtained from the mesenteric artery, hepatic portal vein and the hepatic vein of steers (n = 6) to demonstrate a NMR-based metabolomic approach to study splanchnic metabolism. The metabolic differences between the different plasma samples (arterial, portal, hepatic) were examined by partial least square regressions (PLSR) on the obtained spectra. These analyses revealed spectral differences that could be ascribed to changes in the level of propionate, $-hydroxybutyrate, lactate, acetate, n-butyrate and glucose, which was supported by biochemical analyses.

KW - Metabolism

KW - Metabolomics

KW - NMR

KW - Plasma

KW - Splanchnic

KW - Steer

U2 - 10.22358/jafs/73921/2004

DO - 10.22358/jafs/73921/2004

M3 - Journal article

VL - 13

JO - Journal of Animal and Feed Sciences

JF - Journal of Animal and Feed Sciences

IS - SUPPL. 1

ER -