An Experimental Protocol for Maternal Pulmonary Exposure in Developmental Toxicology

Petra Jackson, Søren P. Lund, Gitte Kristiansen, Ole Andersen, Ulla Vogel, Håkan Wallin, Karin S. Hougaard

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    To establish a protocol for studying effects of pulmonary exposure in developmental toxicity studies, the effects of intratracheal sham instillation under short-term isoflurane anaesthesia were evaluated with a protocol including multiple instillations during gestation. Twelve time-mated mice (C57BL/6BomTac) were anaesthetized with isoflurane and intratracheally instilled with saline containing 10% bronchoalveolar lavage (BAL) on gestation days 8, 11, 15 and 18. In addition, the early effects of the procedure were assessed in naive female mice. Control animals were not handled. Dams were followed until weaning, and the offspring were observed from birth to sexual maturation. The cell composition of BAL was examined in the females early after treatment (3 days) and in the dams at weaning (25 days). DNA damage in BAL and liver cells was determined by the comet assay. The procedure did not affect gestation or viability, growth and sexual maturation of the offspring. Lung markers of inflammation and DNA damage were comparable in control and treated dams. Livers of the anaesthetized and instilled females, dams and their offspring displayed no induction of DNA damage. Intratracheal instillation under isoflurane anaesthesia did not induce observable effects in pregnant mice or their offspring. We suggest that this procedure can be used as a means of exposure through the airways in studies of developmental toxicity.
    OriginalsprogEngelsk
    TidsskriftBasic & Clinical Pharmacology & Toxicology
    Vol/bind108
    Udgave nummer3
    Sider (fra-til)202-207
    ISSN1742-7835
    DOI
    StatusUdgivet - 2011

    Citer dette

    Jackson, P., Lund, S. P., Kristiansen, G., Andersen, O., Vogel, U., Wallin, H., & Hougaard, K. S. (2011). An Experimental Protocol for Maternal Pulmonary Exposure in Developmental Toxicology. Basic & Clinical Pharmacology & Toxicology, 108(3), 202-207 . https://doi.org/10.1111/j.1742-7843.2010.00644.x
    Jackson, Petra ; Lund, Søren P. ; Kristiansen, Gitte ; Andersen, Ole ; Vogel, Ulla ; Wallin, Håkan ; Hougaard, Karin S. / An Experimental Protocol for Maternal Pulmonary Exposure in Developmental Toxicology. I: Basic & Clinical Pharmacology & Toxicology. 2011 ; Bind 108, Nr. 3. s. 202-207 .
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    abstract = "To establish a protocol for studying effects of pulmonary exposure in developmental toxicity studies, the effects of intratracheal sham instillation under short-term isoflurane anaesthesia were evaluated with a protocol including multiple instillations during gestation. Twelve time-mated mice (C57BL/6BomTac) were anaesthetized with isoflurane and intratracheally instilled with saline containing 10{\%} bronchoalveolar lavage (BAL) on gestation days 8, 11, 15 and 18. In addition, the early effects of the procedure were assessed in naive female mice. Control animals were not handled. Dams were followed until weaning, and the offspring were observed from birth to sexual maturation. The cell composition of BAL was examined in the females early after treatment (3 days) and in the dams at weaning (25 days). DNA damage in BAL and liver cells was determined by the comet assay. The procedure did not affect gestation or viability, growth and sexual maturation of the offspring. Lung markers of inflammation and DNA damage were comparable in control and treated dams. Livers of the anaesthetized and instilled females, dams and their offspring displayed no induction of DNA damage. Intratracheal instillation under isoflurane anaesthesia did not induce observable effects in pregnant mice or their offspring. We suggest that this procedure can be used as a means of exposure through the airways in studies of developmental toxicity.",
    author = "Petra Jackson and Lund, {S{\o}ren P.} and Gitte Kristiansen and Ole Andersen and Ulla Vogel and H{\aa}kan Wallin and Hougaard, {Karin S.}",
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    Jackson, P, Lund, SP, Kristiansen, G, Andersen, O, Vogel, U, Wallin, H & Hougaard, KS 2011, 'An Experimental Protocol for Maternal Pulmonary Exposure in Developmental Toxicology' Basic & Clinical Pharmacology & Toxicology, bind 108, nr. 3, s. 202-207 . https://doi.org/10.1111/j.1742-7843.2010.00644.x

    An Experimental Protocol for Maternal Pulmonary Exposure in Developmental Toxicology. / Jackson, Petra; Lund, Søren P.; Kristiansen, Gitte ; Andersen, Ole; Vogel, Ulla; Wallin, Håkan; Hougaard, Karin S.

    I: Basic & Clinical Pharmacology & Toxicology, Bind 108, Nr. 3, 2011, s. 202-207 .

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - An Experimental Protocol for Maternal Pulmonary Exposure in Developmental Toxicology

    AU - Jackson, Petra

    AU - Lund, Søren P.

    AU - Kristiansen, Gitte

    AU - Andersen, Ole

    AU - Vogel, Ulla

    AU - Wallin, Håkan

    AU - Hougaard, Karin S.

    PY - 2011

    Y1 - 2011

    N2 - To establish a protocol for studying effects of pulmonary exposure in developmental toxicity studies, the effects of intratracheal sham instillation under short-term isoflurane anaesthesia were evaluated with a protocol including multiple instillations during gestation. Twelve time-mated mice (C57BL/6BomTac) were anaesthetized with isoflurane and intratracheally instilled with saline containing 10% bronchoalveolar lavage (BAL) on gestation days 8, 11, 15 and 18. In addition, the early effects of the procedure were assessed in naive female mice. Control animals were not handled. Dams were followed until weaning, and the offspring were observed from birth to sexual maturation. The cell composition of BAL was examined in the females early after treatment (3 days) and in the dams at weaning (25 days). DNA damage in BAL and liver cells was determined by the comet assay. The procedure did not affect gestation or viability, growth and sexual maturation of the offspring. Lung markers of inflammation and DNA damage were comparable in control and treated dams. Livers of the anaesthetized and instilled females, dams and their offspring displayed no induction of DNA damage. Intratracheal instillation under isoflurane anaesthesia did not induce observable effects in pregnant mice or their offspring. We suggest that this procedure can be used as a means of exposure through the airways in studies of developmental toxicity.

    AB - To establish a protocol for studying effects of pulmonary exposure in developmental toxicity studies, the effects of intratracheal sham instillation under short-term isoflurane anaesthesia were evaluated with a protocol including multiple instillations during gestation. Twelve time-mated mice (C57BL/6BomTac) were anaesthetized with isoflurane and intratracheally instilled with saline containing 10% bronchoalveolar lavage (BAL) on gestation days 8, 11, 15 and 18. In addition, the early effects of the procedure were assessed in naive female mice. Control animals were not handled. Dams were followed until weaning, and the offspring were observed from birth to sexual maturation. The cell composition of BAL was examined in the females early after treatment (3 days) and in the dams at weaning (25 days). DNA damage in BAL and liver cells was determined by the comet assay. The procedure did not affect gestation or viability, growth and sexual maturation of the offspring. Lung markers of inflammation and DNA damage were comparable in control and treated dams. Livers of the anaesthetized and instilled females, dams and their offspring displayed no induction of DNA damage. Intratracheal instillation under isoflurane anaesthesia did not induce observable effects in pregnant mice or their offspring. We suggest that this procedure can be used as a means of exposure through the airways in studies of developmental toxicity.

    U2 - 10.1111/j.1742-7843.2010.00644.x

    DO - 10.1111/j.1742-7843.2010.00644.x

    M3 - Journal article

    VL - 108

    SP - 202

    EP - 207

    JO - Basic & Clinical Pharmacology & Toxicology

    JF - Basic & Clinical Pharmacology & Toxicology

    SN - 1742-7835

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    ER -