A simple and fast method for extraction and quantification of cryptophyte phycoerythrin

Publikation: Bidrag til tidsskriftTidsskriftartikel

Resumé

The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.

•Simple method for extraction and quantification of phycoerythrin from cryptophytes.
•Minimal usage of equipment and chemicals, and low labor costs.
•Applicable for industrial and biological purposes.
The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.

•Simple method for extraction and quantification of phycoerythrin from cryptophytes.
•Minimal usage of equipment and chemicals, and low labor costs.
•Applicable for industrial and biological purposes.
SprogEngelsk
TidsskriftMethodsX
Vol/bind4
Udgave nummerC
Sider209-213
Antal sider5
ISSN2215-0161
DOI
StatusUdgivet - 28 jul. 2017

Emneord

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    title = "A simple and fast method for extraction and quantification of cryptophyte phycoerythrin",
    abstract = "The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.•Simple method for extraction and quantification of phycoerythrin from cryptophytes.•Minimal usage of equipment and chemicals, and low labor costs.•Applicable for industrial and biological purposes.",
    keywords = "Labor cost, Microalgae, Phycobiliprotein, Pigment, Processing time, Rhodomonas salina",
    author = "Thoisen, {Christina Vinum} and Hansen, {Benni Winding} and Nielsen, {S\{o}ren Laurentius}",
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    AU - Thoisen,Christina Vinum

    AU - Hansen,Benni Winding

    AU - Nielsen,Søren Laurentius

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    AB - The microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. Filters with the cryptophyte were frozen (−80 °C) and added phosphate buffer for extraction at 4 °C followed by absorbance measurement. The cryptophyte Rhodomonas salina was used as a model organism.•Simple method for extraction and quantification of phycoerythrin from cryptophytes.•Minimal usage of equipment and chemicals, and low labor costs.•Applicable for industrial and biological purposes.

    KW - Labor cost

    KW - Microalgae

    KW - Phycobiliprotein

    KW - Pigment

    KW - Processing time

    KW - Rhodomonas salina

    U2 - https://doi.org/10.1016/j.mex.2017.06.002

    DO - https://doi.org/10.1016/j.mex.2017.06.002

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    SP - 209

    EP - 213

    JO - MethodsX

    T2 - MethodsX

    JF - MethodsX

    SN - 2215-0161

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