A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice

Maryjane Utley; David P Franklin; Karen A Krogfelt; David C Laux; Paul S Cohen

doi:10.1111/j.1574-6968.1998.tb13036.x

A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice

Maryjane Utley, David P Franklin, Karen A Krogfelt, David C Laux, Paul S Cohen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Salmonella typhimurium SR-11 is extremely virulent at a dose as low as 105 colony forming units (cfu) when administered perorally to BALB/c mice. Utilizing mini-transposon mutagenesis, a mutant of S. typhimurium SR-11 was isolated that was unable to utilize oleate and citrate as carbon sources. This mutant, designated S. typhimurium SR-11 Fad− (Fatty acid), was found to utilize sugars under cya/crp control as sole carbon sources, suggesting that the mutation is not in either of these genes. In addition, SR-11 Fad− utilized pyruvate and succinate, but was unable to utilize either acetate or isocitrate as sole carbon source. In contrast to SR-11, SR-11 Fad− was found to be avirulent, i.e. BALB/c mice were completely healthy after oral infection with 109S. typhimurium SR-11 Fad− cells. Moreover, 21 days after SR-11 Fad− infection, BALB/c mice were found to be protected against an oral challenge with 109 cells of S. typhimurium SR-11.

Originalsprog	Engelsk
Tidsskrift	FEMS Microbiology Letters
Vol/bind	163
Udgave nummer	2
Sider (fra-til)	129-134
Antal sider	6
ISSN	0378-1097
DOI	https://doi.org/10.1111/j.1574-6968.1998.tb13036.x
Status	Udgivet - 1998
Udgivet eksternt	Ja

Link til dokument

10.1111/j.1574-6968.1998.tb13036.x

Citer dette

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title = "A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice",

abstract = "Salmonella typhimurium SR-11 is extremely virulent at a dose as low as 105 colony forming units (cfu) when administered perorally to BALB/c mice. Utilizing mini-transposon mutagenesis, a mutant of S. typhimurium SR-11 was isolated that was unable to utilize oleate and citrate as carbon sources. This mutant, designated S. typhimurium SR-11 Fad− (Fatty acid), was found to utilize sugars under cya/crp control as sole carbon sources, suggesting that the mutation is not in either of these genes. In addition, SR-11 Fad− utilized pyruvate and succinate, but was unable to utilize either acetate or isocitrate as sole carbon source. In contrast to SR-11, SR-11 Fad− was found to be avirulent, i.e. BALB/c mice were completely healthy after oral infection with 109S. typhimurium SR-11 Fad− cells. Moreover, 21 days after SR-11 Fad− infection, BALB/c mice were found to be protected against an oral challenge with 109 cells of S. typhimurium SR-11.",

author = "Maryjane Utley and Franklin, {David P} and Krogfelt, {Karen A} and Laux, {David C} and Cohen, {Paul S}",

year = "1998",

doi = "10.1111/j.1574-6968.1998.tb13036.x",

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journal = "FEMS Microbiology Letters",

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TY - JOUR

T1 - A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice

AU - Utley, Maryjane

AU - Franklin, David P

AU - Krogfelt, Karen A

AU - Laux, David C

AU - Cohen, Paul S

PY - 1998

Y1 - 1998

N2 - Salmonella typhimurium SR-11 is extremely virulent at a dose as low as 105 colony forming units (cfu) when administered perorally to BALB/c mice. Utilizing mini-transposon mutagenesis, a mutant of S. typhimurium SR-11 was isolated that was unable to utilize oleate and citrate as carbon sources. This mutant, designated S. typhimurium SR-11 Fad− (Fatty acid), was found to utilize sugars under cya/crp control as sole carbon sources, suggesting that the mutation is not in either of these genes. In addition, SR-11 Fad− utilized pyruvate and succinate, but was unable to utilize either acetate or isocitrate as sole carbon source. In contrast to SR-11, SR-11 Fad− was found to be avirulent, i.e. BALB/c mice were completely healthy after oral infection with 109S. typhimurium SR-11 Fad− cells. Moreover, 21 days after SR-11 Fad− infection, BALB/c mice were found to be protected against an oral challenge with 109 cells of S. typhimurium SR-11.

AB - Salmonella typhimurium SR-11 is extremely virulent at a dose as low as 105 colony forming units (cfu) when administered perorally to BALB/c mice. Utilizing mini-transposon mutagenesis, a mutant of S. typhimurium SR-11 was isolated that was unable to utilize oleate and citrate as carbon sources. This mutant, designated S. typhimurium SR-11 Fad− (Fatty acid), was found to utilize sugars under cya/crp control as sole carbon sources, suggesting that the mutation is not in either of these genes. In addition, SR-11 Fad− utilized pyruvate and succinate, but was unable to utilize either acetate or isocitrate as sole carbon source. In contrast to SR-11, SR-11 Fad− was found to be avirulent, i.e. BALB/c mice were completely healthy after oral infection with 109S. typhimurium SR-11 Fad− cells. Moreover, 21 days after SR-11 Fad− infection, BALB/c mice were found to be protected against an oral challenge with 109 cells of S. typhimurium SR-11.

U2 - 10.1111/j.1574-6968.1998.tb13036.x

DO - 10.1111/j.1574-6968.1998.tb13036.x

M3 - Journal article

SN - 0378-1097

VL - 163

SP - 129

EP - 134

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -