A novel MLL-AF10 fusion mRNA variant in a patient with acute myeloid leukemia detected by a new asymmetric reverse transcription PCR method

B. Hjorth-Sørensen, N. Pallisgaard, M. Grönholm, P. Hokland, N. Clausen, P. Jørgensen*

*Corresponding author

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Abstract

A novel variant of the chimerical MLL-AF10 mRNA transcript was detected in a pediatric patient with acute myeloid leukemia (AML) by a new asymmetric reverse-transcription polymerase chain reaction (ART-PCR) method. Sequence analysis of the fusion region on the amplified cDNA fragment showed an in-frame joining of exon e5 of the MLL gene and position 1931 of the cDNA sequence of the AF10 gene, giving rise to a new MLL-AF10 transcript. The presence of the new chimerical mRNA product in a sample from the patient was confirmed by classical RT-PCR.

OriginalsprogEngelsk
TidsskriftLeukemia
Vol/bind11
Udgave nummer9
Sider (fra-til)1588-1593
Antal sider6
ISSN0887-6924
DOI
StatusUdgivet - 1997
Udgivet eksterntJa

Emneord

  • Acute myeloid leukemia
  • Fusion mRNA
  • MLL-AF10

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