A Multimethod Approach for Investigating Algal Toxicity of Platinum Nanoparticles

Sara Nørgaard Sørensen, Christian Engelbrekt, Hans-Christian Holten Lützhøft, Javier Jiménez-Lamana, Jafar Safaa Noori, Fatima AlZahraa Alatraktchi, Cristina Giron Delgado, Vera I. Slaveykova, Anders Baun

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The ecotoxicity of platinum nanoparticles (PtNPs) widely used in for example automotive catalytic converters, is largely unknown. This study employs various characterization techniques and toxicity end points to investigate PtNP toxicity toward the green microalgae Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. Growth rate inhibition occurred in standard ISO tests (EC50 values of 15–200 mg Pt/L), but also in a double-vial setup, separating cells from PtNPs, thus demonstrating shading as an important artifact for PtNP toxicity. Negligible membrane damage, but substantial oxidative stress was detected at 0.1–80 mg Pt/L in both algal species using flow cytometry. PtNPs caused growth rate inhibition and oxidative stress in P. subcapitata, beyond what was accounted for by dissolved Pt, indicating NP-specific toxicity of PtNPs. Overall, P. subcapitata was found to be more sensitive toward PtNPs and higher body burdens were measured in this species, possibly due to a favored binding of Pt to the polysaccharide-rich cell wall of this algal species. This study highlights the importance of using multimethod approaches in nanoecotoxicological studies to elucidate toxicity mechanisms, influence of NP-interactions with media/organisms, and ultimately to identify artifacts and appropriate end points for NP-ecotoxicity testing.
OriginalsprogEngelsk
TidsskriftEnvironmental Science & Technology (Washington)
Vol/bind50
Udgave nummer19
Sider (fra-til)10635-10643
ISSN0013-936X
DOI
StatusUdgivet - 2016
Udgivet eksterntJa

Citer dette

Sørensen, S. N., Engelbrekt, C., Lützhøft, H-C. H., Jiménez-Lamana, J., Noori, J. S., Alatraktchi, F. A., ... Baun, A. (2016). A Multimethod Approach for Investigating Algal Toxicity of Platinum Nanoparticles. Environmental Science & Technology (Washington), 50(19), 10635-10643. https://doi.org/10.1021/acs.est.6b01072
Sørensen, Sara Nørgaard ; Engelbrekt, Christian ; Lützhøft, Hans-Christian Holten ; Jiménez-Lamana, Javier ; Noori, Jafar Safaa ; Alatraktchi, Fatima AlZahraa ; Giron Delgado, Cristina ; Slaveykova, Vera I. ; Baun, Anders. / A Multimethod Approach for Investigating Algal Toxicity of Platinum Nanoparticles. I: Environmental Science & Technology (Washington). 2016 ; Bind 50, Nr. 19. s. 10635-10643.
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title = "A Multimethod Approach for Investigating Algal Toxicity of Platinum Nanoparticles",
abstract = "The ecotoxicity of platinum nanoparticles (PtNPs) widely used in for example automotive catalytic converters, is largely unknown. This study employs various characterization techniques and toxicity end points to investigate PtNP toxicity toward the green microalgae Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. Growth rate inhibition occurred in standard ISO tests (EC50 values of 15\200 mg Pt/L), but also in a double-vial setup, separating cells from PtNPs, thus demonstrating shading as an important artifact for PtNP toxicity. Negligible membrane damage, but substantial oxidative stress was detected at 0.1\80 mg Pt/L in both algal species using flow cytometry. PtNPs caused growth rate inhibition and oxidative stress in P. subcapitata, beyond what was accounted for by dissolved Pt, indicating NP-specific toxicity of PtNPs. Overall, P. subcapitata was found to be more sensitive toward PtNPs and higher body burdens were measured in this species, possibly due to a favored binding of Pt to the polysaccharide-rich cell wall of this algal species. This study highlights the importance of using multimethod approaches in nanoecotoxicological studies to elucidate toxicity mechanisms, influence of NP-interactions with media/organisms, and ultimately to identify artifacts and appropriate end points for NP-ecotoxicity testing.",
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Sørensen, SN, Engelbrekt, C, Lützhøft, H-CH, Jiménez-Lamana, J, Noori, JS, Alatraktchi, FA, Giron Delgado, C, Slaveykova, VI & Baun, A 2016, 'A Multimethod Approach for Investigating Algal Toxicity of Platinum Nanoparticles' Environmental Science & Technology (Washington), bind 50, nr. 19, s. 10635-10643. https://doi.org/10.1021/acs.est.6b01072

A Multimethod Approach for Investigating Algal Toxicity of Platinum Nanoparticles. / Sørensen, Sara Nørgaard; Engelbrekt, Christian; Lützhøft, Hans-Christian Holten; Jiménez-Lamana, Javier; Noori, Jafar Safaa; Alatraktchi, Fatima AlZahraa; Giron Delgado, Cristina; Slaveykova, Vera I.; Baun, Anders.

I: Environmental Science & Technology (Washington), Bind 50, Nr. 19, 2016, s. 10635-10643.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - A Multimethod Approach for Investigating Algal Toxicity of Platinum Nanoparticles

AU - Sørensen, Sara Nørgaard

AU - Engelbrekt, Christian

AU - Lützhøft, Hans-Christian Holten

AU - Jiménez-Lamana, Javier

AU - Noori, Jafar Safaa

AU - Alatraktchi, Fatima AlZahraa

AU - Giron Delgado, Cristina

AU - Slaveykova, Vera I.

AU - Baun, Anders

PY - 2016

Y1 - 2016

N2 - The ecotoxicity of platinum nanoparticles (PtNPs) widely used in for example automotive catalytic converters, is largely unknown. This study employs various characterization techniques and toxicity end points to investigate PtNP toxicity toward the green microalgae Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. Growth rate inhibition occurred in standard ISO tests (EC50 values of 15\200 mg Pt/L), but also in a double-vial setup, separating cells from PtNPs, thus demonstrating shading as an important artifact for PtNP toxicity. Negligible membrane damage, but substantial oxidative stress was detected at 0.1\80 mg Pt/L in both algal species using flow cytometry. PtNPs caused growth rate inhibition and oxidative stress in P. subcapitata, beyond what was accounted for by dissolved Pt, indicating NP-specific toxicity of PtNPs. Overall, P. subcapitata was found to be more sensitive toward PtNPs and higher body burdens were measured in this species, possibly due to a favored binding of Pt to the polysaccharide-rich cell wall of this algal species. This study highlights the importance of using multimethod approaches in nanoecotoxicological studies to elucidate toxicity mechanisms, influence of NP-interactions with media/organisms, and ultimately to identify artifacts and appropriate end points for NP-ecotoxicity testing.

AB - The ecotoxicity of platinum nanoparticles (PtNPs) widely used in for example automotive catalytic converters, is largely unknown. This study employs various characterization techniques and toxicity end points to investigate PtNP toxicity toward the green microalgae Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. Growth rate inhibition occurred in standard ISO tests (EC50 values of 15\200 mg Pt/L), but also in a double-vial setup, separating cells from PtNPs, thus demonstrating shading as an important artifact for PtNP toxicity. Negligible membrane damage, but substantial oxidative stress was detected at 0.1\80 mg Pt/L in both algal species using flow cytometry. PtNPs caused growth rate inhibition and oxidative stress in P. subcapitata, beyond what was accounted for by dissolved Pt, indicating NP-specific toxicity of PtNPs. Overall, P. subcapitata was found to be more sensitive toward PtNPs and higher body burdens were measured in this species, possibly due to a favored binding of Pt to the polysaccharide-rich cell wall of this algal species. This study highlights the importance of using multimethod approaches in nanoecotoxicological studies to elucidate toxicity mechanisms, influence of NP-interactions with media/organisms, and ultimately to identify artifacts and appropriate end points for NP-ecotoxicity testing.

U2 - 10.1021/acs.est.6b01072

DO - 10.1021/acs.est.6b01072

M3 - Journal article

VL - 50

SP - 10635

EP - 10643

JO - Environmental Science & Technology (Washington)

JF - Environmental Science & Technology (Washington)

SN - 0013-936X

IS - 19

ER -