A method for studying the metabolic activity of individual tardigrades by measuring oxygen uptake using micro-respirometry

Bjarke H. Pedersen, Hans Malte, Hans Ramløv*, Kai Finster

*Corresponding author

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Studies of tardigrade biology have been severely limited by the sparsity of appropriate quantitative techniques, informative on a single-organism level. Therefore, many studies rely on motility-based survival scoring and quantifying reproductive success. Measurements of O2 respiration rates, as an integrating expression of the metabolic activity of single tardigrades, would provide a more comprehensive insight into how an individual tardigrade is responding to specific environmental factors or changes in life stages. Here we present and validate a new method for determining the O2 respiration rate (nmol O2 mg−1 hour−1) of single tardigrades under steady state, using O2-microsensors. As an example, we show that the O2 respiration rate determined in MilliQ water for individuals of Richtersius coronifer and of Macrobiotus macrocalix at 22 °C was 10.8±1.8 nmol O2 mg−1 hour−1 and 13.1±2.3 nmol O2 mg−1 hour−1, respectively.
TidsskriftJournal of Experimental Biology
Udgave nummer22
StatusUdgivet - 19 nov. 2020


  • Energetics
  • O2 respiration rate
  • Metabolism
  • Tardigrada

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