A GC-MS-based metabonomic investigation of blood serum from irritable bowel syndrome patients undergoing intervention with acidified milk products

S.M.M. Pedersen, C. Nebel, N.C. Nielsen, H.J. Andersen, J. Olsson, M. Simrén, L. Öhman, U. Svensson, H.C. Bertram, A. Malmendal

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In the present study, the use of gas chromatography mass spectrometry (GC-MS)-based metabonomics to characterize blood serum in an intervention study of patients suffering from the common gastrointestinal disorder irritable bowel syndrome (IBS) was investigated. The patients included in the study consumed an acidified milk product with (n = 30) or without probiotics (n = 31) (Lactobacillus paracasei F19, Lactobacillus acidophilus LA-5 and Bifidobacterium lactis BB-12) for an 8-week period, and blood serum samples were collected before and after the intervention. Acidified milk is commonly used as a delivering vector for probiotics in commercial consumer settings. The serum samples were extracted and derivatized using N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA), and GC-MS analysis was carried out. Multivariate data analysis including principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and S-plot was applied on the obtained GC-MS data, which revealed higher serum lactate, glutamine, proline creatinine/creatine, and aspartic acid levels and lower serum glucose levels after the intervention period for both treatment groups. Consequently, the present study indicated an effect of acidified milk consumption on the plasma metabolite profile, which was independent of a concomitant intake of probiotics. In addition, the present study demonstrates that GC-MS is a useful analytical technique for metabonomics studies of blood serum. textcopyright 2011 Springer-Verlag.
TidsskriftEuropean Food Research and Technology
Udgave nummer6
StatusUdgivet - 2011


  • Acidified milk products
  • Derivatization
  • GC-MS
  • Irritable bowel syndrome
  • Lactate
  • Metabonomics
  • Probiotics

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