A fast and easy real-time PCR genotyping method for the HLA-G 14-bp insertion/deletion polymorphism in the 3 untranslated region

S. Djurisic, Anja Elaine Sørensen, T. V. F. Hviid

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Abstract

    Human leukocyte antigen-G (HLA-G) is a non-classical HLA class Ib molecule shown to exhibit immunomodulatory function in a wide range of immune-based disorders. A number of functional HLA-G gene polymorphisms have been identified, including a 14-bp insertion/deletion polymorphism in exon 8 of the 3 untranslated region of the HLA-G gene, which has been associated with HLA-G mRNA stability. Moreover, studies show that homozygosity for the 14-bp insertion/deletion polymorphism is associated with lower HLA-G mRNA and protein levels and unique alternative splicing patterns. Here, we introduce a quick and reliable method to screen for the HLA-G 14-bp insertion/deletion polymorphism using an optimized real-time polymerase chain reaction protocol. The genotyping assay has been validated by comparison with conventional methods. As results can be obtained within a few hours, the assay will have a potential for clinical use.
    OriginalsprogEngelsk
    TidsskriftHLA
    Vol/bind79
    Udgave nummer3
    Sider (fra-til)186-189
    ISSN2059-2302
    DOI
    StatusUdgivet - 2012

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