A Drosophila Genome-Wide Screen Identifies Regulators of Steroid Hormone Production and Developmental Timing

E. Thomas Danielsen, Morten E. Moeller, Naoki Yamanaka, Qiuxiang Ou, Janne M. Laursen, Caecilie Soenderholm, Ran Zhuo, Brian Phelps, Kevin Tang, Jie Zeng, Shu Kondo, Christian H. Nielsen, Eva B. Harvald, Nils J. Faergeman, Macy J. Haley, Kyle A. O'Connor, Kirst King-Jones, Michael B. O'Connor, Kim F. Rewitz

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Abstract

Steroid hormones control important developmental processes and are linked to many diseases. To systematically identify genes and pathways required for steroid production, we performed a Drosophila genome-wide in vivo RNAi screen and identified 1,906 genes with potential roles in steroidogenesis and developmental timing. Here, we use our screen as a resource to identify mechanisms regulating intracellular levels of cholesterol, a substrate for steroidogenesis. We identify a conserved fatty acid elongase that underlies a mechanism that adjusts cholesterol trafficking and steroidogenesis with nutrition and developmental programs. In addition, we demonstrate the existence of an autophagosomal cholesterol mobilization mechanism and show that activation of this system rescues Niemann-Pick type C1 deficiency that causes a disorder characterized by cholesterol accumulation. These cholesterol-trafficking mechanisms are regulated by TOR and feedback signaling that couples steroidogenesis with growth and ensures proper maturation timing. These results reveal genes regulating steroidogenesis during development that likely modulate disease mechanisms.
OriginalsprogEngelsk
TidsskriftDevelopmental Cell
Vol/bind37
Udgave nummer6
Sider (fra-til)558-570
Antal sider13
ISSN1534-5807
DOI
StatusUdgivet - 2016
Udgivet eksterntJa

Citer dette

Danielsen, E. T., Moeller, M. E., Yamanaka, N., Ou, Q., Laursen, J. M., Soenderholm, C., Zhuo, R., Phelps, B., Tang, K., Zeng, J., Kondo, S., Nielsen, C. H., Harvald, E. B., Faergeman, N. J., Haley, M. J., O'Connor, K. A., King-Jones, K., O'Connor, M. B., & Rewitz, K. F. (2016). A Drosophila Genome-Wide Screen Identifies Regulators of Steroid Hormone Production and Developmental Timing. Developmental Cell, 37(6), 558-570. https://doi.org/10.1016/j.devcel.2016.05.015