1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor

Zhanna Tairova, Anders Giessing, Rikke Hansen, Ole Andersen

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    The possibility of using the pyrene metabolite I-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons (PAHs) exposure was investigated by exposure of the marine polychaete Nereis diversicolor to several PAHs in the laboratory. Animals were exposed to pyrene alone and to five different PAHs phenanthrene, anthracene, pyrene, benzo[a]pyrene and benzo[k]flouranthene. After five days of exposure the concentrations of parent PAHs and 1-hydroxypyrene were identified using three different analytical methods, high-performance liquid chromatography with fluorescence detection (HPLC/F), synchronous fluorescence spectroscopy (SFS) and gas chromatography with mass spectrometric detection (GC/MS). The 5FS measurements of 1-hydroxypyrene were validated by the more sensitive method HPLC/F. The positive correlation between total PAHs and 1-hydroxypyrene concentrations in the polychaete tissues observed in experiments, suggests the feasibility of 1-hydroxypyrene as a suitable biomarker for total PAH exposure assessment. Furthermore, the possibility of employment of the simple and rapid SFS method instead of HPLC/F for biomarker analysis has been confirmed by the positive and significant correlation between results achieved by these two analytical methods.
    OriginalsprogEngelsk
    TidsskriftMarine Environmental Research
    Vol/bind67
    Udgave nummer1
    Sider (fra-til)38-46
    ISSN0141-1136
    DOI
    StatusUdgivet - 2009

    Citer dette

    Tairova, Zhanna ; Giessing, Anders ; Hansen, Rikke ; Andersen, Ole. / 1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor. I: Marine Environmental Research. 2009 ; Bind 67, Nr. 1. s. 38-46.
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    title = "1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor",
    abstract = "The possibility of using the pyrene metabolite I-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons (PAHs) exposure was investigated by exposure of the marine polychaete Nereis diversicolor to several PAHs in the laboratory. Animals were exposed to pyrene alone and to five different PAHs phenanthrene, anthracene, pyrene, benzo[a]pyrene and benzo[k]flouranthene. After five days of exposure the concentrations of parent PAHs and 1-hydroxypyrene were identified using three different analytical methods, high-performance liquid chromatography with fluorescence detection (HPLC/F), synchronous fluorescence spectroscopy (SFS) and gas chromatography with mass spectrometric detection (GC/MS). The 5FS measurements of 1-hydroxypyrene were validated by the more sensitive method HPLC/F. The positive correlation between total PAHs and 1-hydroxypyrene concentrations in the polychaete tissues observed in experiments, suggests the feasibility of 1-hydroxypyrene as a suitable biomarker for total PAH exposure assessment. Furthermore, the possibility of employment of the simple and rapid SFS method instead of HPLC/F for biomarker analysis has been confirmed by the positive and significant correlation between results achieved by these two analytical methods.",
    author = "Zhanna Tairova and Anders Giessing and Rikke Hansen and Ole Andersen",
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    doi = "10.1016/j.marenvres.2008.10.005",
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    1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor. / Tairova, Zhanna; Giessing, Anders; Hansen, Rikke; Andersen, Ole.

    I: Marine Environmental Research, Bind 67, Nr. 1, 2009, s. 38-46.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - 1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor

    AU - Tairova, Zhanna

    AU - Giessing, Anders

    AU - Hansen, Rikke

    AU - Andersen, Ole

    PY - 2009

    Y1 - 2009

    N2 - The possibility of using the pyrene metabolite I-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons (PAHs) exposure was investigated by exposure of the marine polychaete Nereis diversicolor to several PAHs in the laboratory. Animals were exposed to pyrene alone and to five different PAHs phenanthrene, anthracene, pyrene, benzo[a]pyrene and benzo[k]flouranthene. After five days of exposure the concentrations of parent PAHs and 1-hydroxypyrene were identified using three different analytical methods, high-performance liquid chromatography with fluorescence detection (HPLC/F), synchronous fluorescence spectroscopy (SFS) and gas chromatography with mass spectrometric detection (GC/MS). The 5FS measurements of 1-hydroxypyrene were validated by the more sensitive method HPLC/F. The positive correlation between total PAHs and 1-hydroxypyrene concentrations in the polychaete tissues observed in experiments, suggests the feasibility of 1-hydroxypyrene as a suitable biomarker for total PAH exposure assessment. Furthermore, the possibility of employment of the simple and rapid SFS method instead of HPLC/F for biomarker analysis has been confirmed by the positive and significant correlation between results achieved by these two analytical methods.

    AB - The possibility of using the pyrene metabolite I-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons (PAHs) exposure was investigated by exposure of the marine polychaete Nereis diversicolor to several PAHs in the laboratory. Animals were exposed to pyrene alone and to five different PAHs phenanthrene, anthracene, pyrene, benzo[a]pyrene and benzo[k]flouranthene. After five days of exposure the concentrations of parent PAHs and 1-hydroxypyrene were identified using three different analytical methods, high-performance liquid chromatography with fluorescence detection (HPLC/F), synchronous fluorescence spectroscopy (SFS) and gas chromatography with mass spectrometric detection (GC/MS). The 5FS measurements of 1-hydroxypyrene were validated by the more sensitive method HPLC/F. The positive correlation between total PAHs and 1-hydroxypyrene concentrations in the polychaete tissues observed in experiments, suggests the feasibility of 1-hydroxypyrene as a suitable biomarker for total PAH exposure assessment. Furthermore, the possibility of employment of the simple and rapid SFS method instead of HPLC/F for biomarker analysis has been confirmed by the positive and significant correlation between results achieved by these two analytical methods.

    U2 - 10.1016/j.marenvres.2008.10.005

    DO - 10.1016/j.marenvres.2008.10.005

    M3 - Journal article

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    SP - 38

    EP - 46

    JO - Marine Environmental Research

    JF - Marine Environmental Research

    SN - 0141-1136

    IS - 1

    ER -