Foto af Louise Torp Dalgaard
  • Universitetsvej 1, 28A.1

    DK-4000 Roskilde

    Danmark

19972018
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Personlig profil

Forsknings og undervisningsoplysninger

Lecturer on the following courses:

Human biology and evolution (introductory course)

Basic eucaryotic cell biology

Advanced eucaryotic cell biology

Genetics

Immunology

Seminar course in Diabetes Mellitus and Beta-cell Biology

Experimental Cell Biology

Projects in Molecular Biology and Medicinal Biology 

 

Course administrator for the following courses:

Immunology

Seminar course in Diabetes Mellitus and Beta-cell Biology 

Experimental Cell Biology

Forskningsområder

The research interests of the Diabetes and β-cell biology Group focuses (not surprisingly) on diabetes mellitus and the insulin secreting β-cell, which is located in the Islets of Langerhans in the pancreas. The lab has several projects that focus on the molecular events leading to β-cell failure during development of Type 2 diabetes mellitus. We are also generally interested in mechanisms in cell or molecular biology involved in development of diabetes mellitus and its complications.

MicroRNA is another research interest of the lab. MicroRNAs are tiny 21-23bp single-stranded RNAs that the cell endogenously express. The microRNA is transcribed as a longer precursor with a stem loop structure. Subsequently the miRNA is excised from the stem loop and transported out of the nucleus where it enters the same RISC complex as exogenously added small interfering RNA do. The microRNA then act as a transcriptional or translational repressor by binding to mRNA targets. Complete base pairing between the microRNA and the target mRNA will direct target mRNA degradation, while incomplete base pairing will halt translation of the mRNA. Thus, due to the small size and the possibility of incomplete base pairing a single microRNA may have multiple target mRNAs. MicroRNA thus acts as an extra layer of gene-regulation on top of the normal gene-regulation by transcription factors and cellular signalling.  The lab is interested in the role of miRNAs in β-cell development and β-cell function. We are also involved in projects looking at microRNAs as biomarkers for development of diabetes-related traits.

The role of oxidative stress and anti-oxidative proteins following exposure of β-cells to increased glucose and fatty acid levels is a field of long-time interest. The putative anti-oxidative protein Uncoupling protein 2 is induced by glucose and fatty acids, but this induction causes defective insulin secretion. However, the induction of UCP2, while causing β-cell dysfunction, presumably protects against apoptosis due to oxidative stress. 

The lab uses general molecular biology and cell biology tools to study the questions at hand. These include Q-PCR, cloning (expression, RNAi), northern blotting, western blotting, promoter studies, transfektion of cells. In general we use either isolated islets of Langerhans or a transformed β-cell line (insulinoma cells) for our experiments. Our research typically involves measuring insulin secretion, insulin content, cell survival or apoptosis, ATP content and various other metabolic assays.

Curriculum

Education:

2001    Ph.D., Biomedicine, University of Copenhagen, Denmark. Subject: Genetics of Obesity and Type 2 Diabetes Mellitus. Mentors: Oluf Pedersen and Torben Hansen. Steno Diabetes Center (1997-2001)

1998    M.Sc. Human Biology, Faculty of Health Science, University of Copenhagen (1995-1998). Thesis work at Steno Diabetes Center. Subject: Genetics of Obesity and Type 2 Diabetes Mellitus. Mentor: Oluf Pedersen

1995   Biotechnology and chemistry bachelor studies. University of Aarhus, Denmark (1992-                                   1995)

1992   International Student Program,University of Oklahoma, Oklahoma, USA. Chemistry and biochemistry (1991-1992)

 
Professional experience:

 

2007-p.t.        Associate professor in Medicinal Biology, Dep. of Science, Roskilde University, Denmark

2011-2012     Maternity leave (9 months)

2008-2009     Maternity leave (11 months)

2006-2007     Maternity leave (9 months)

2004-2007     Assistant professor (from  2006 Associate prof.), the Diabetes Group, Dep. of Medical Biochemistry and Genetics, University of Copenhagen.

2003-2004     Research Scientist, Dep. of Virology, Statens Serum Institut, Copenhagen, Denmark. Area of work: Development of real-time PCR-diagnostics for detection of human pathogenic virus.

2001-2003     Post-doctoral fellow in the lab of Dr. Bradford B. Lowell, M.D., Ph.D., Harvard                                                                  Medical School, Boston, MA. Research area: Type 2 diabetes and  obesity.

1997-2001     Ph.d.-studies in the genetics of type 2 diabetes and obesity at DiabetesCenter, Gentofte, Denmark

1994               Summer internship at the Dept. of Bacterial Sceening and Genetics, Novo Nordisk A/S

 

Mentoring and supervising experience

Post-doctoral fellows: 2007-2009 M.W. Rosenstierne (now at ssi.dk), 2013-2016 Sofia Salö

Graduate students: 2 part-time Ph.D.-students (A. Widlund) and (S. Anthonsen), 1 full-time (A Bagge, graduated 2011)

Masters students: M. Elmshøj, S. Anthonsen (continued into Ph.D.),              A.L.Widlund (continued into Ph.D.), M. Møller (continued into Ph.D.), G. Tønnesen, A.E. Sørensen, J. Pedersen, E.P. Jensen, S.S. Nielsen, T.L.Lundstrøm. Advisor for currently 1 student enrolled in experimental master’s studies

Scholar-students: D.B. Jensen (continued into Ph.D.)

                                                                                                                        

Administrative service/Memberships

Member of the Study Board for Biology at Roskilde University

Member of the Danish Ph.D. School of Molecular Metabolism and Endocrinology/Danish Diabetes Academy

Ad hoc reviewer for: Diabetologia, Diabetes, Pflugers Archiv-European Journal of Physiology, AJP- Endocrinology and Metabolism, Hormone and Metabolic Research, Cerebrovascular Diseases, Regulatory peptides, International Journal of Obesity, Human Molecular Genetics, Diabetes & Metabolism, Molecular and Cellular Endocrinology, Journal of Anatomy, Molecular Genetics and Metabolism

Grant reviews: Wellcome Trust, Diabetes UK, The Netherlands Organisation for Health Research and Development, The Polish Science Foundation

 

Grant support:

 

2013-2016   Danish research council for health (FSS) (DKK 2.591.000) for the project “Micro-RNA in mitochondria as molecular targets in metabolism”

2013-2016   Post-doctoral fellowship from the Danish DiabetesAcademy to Sofia Salö (DKK 1.800.000)

2013-2015   Co-investigator on Grant for fertility innovation (GFI, Euro 173.000): “MicroRNAs in biobank serum,
follicular fluid and cumulus cells of women with polycystic ovary syndrome and healthy, regularly cycling women”

2008-2011   Danish research council for technology and production (DKK 2.718.000) for the project ”Development of a new diagnostic test for blood glucose monitoring in diabetic patients – an alternative to the HbA1c test” on identification and characteristics of glucose-regulated microRNA

2006-2008   Member of the Danish Innovation consortia ’Dansk micro-RNA konsortium’, with support of DKK 2.000.000 over 3 years for post doc

2004-2007 Danish Medical Research Council: Research grant for studies at Department of Medical Biochemistry and Genetics at University of Copenhagen (salary and consumables). Other grant support: Novo Nordisk Foundation, Danish Diabetes Assocation, Haensch Foundation, Aase and Ejner Danielsens Foundation

2001-2003 Danish Medical Research Council: Research grant for post-doctoral studies at Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA (salary and travel costs).

1997-2000 Ph.d.-scholarship from University of Copenhagen

1998, 2013                    Poul og Erna Sehested Hansen Fonds Forskningslegat (Research stipend)

1998, 1999, 2000  Travel grants for the European Association of Diabetes meetings from the Danish Diabetes Assocation

1994 Manufacturer Åge Lichtingers Scholarship

1991-1992 Full scholarship from Thanks to Scandinavia Foundation for studies at University of Oklahoma, USA

Keywords

  • Sundhed, Medicin
  • Diabetes mellitus
  • Type 2 diabetes mellitus
  • Type 1 diabetes mellitus
  • Betacelle biologi
  • Betacelle dysfunktion
  • Langerhanske øer
  • Oksidativt stress
  • Mitochondrier
  • Biologi, Bioteknologi
  • Transcriptional biology
  • Intracellulær signalering
  • MicroRNA

Publikationsnetværk Nyligt eksternt samarbejde på landeniveau. Dyk ned i detaljerne ved at klikke på punkterne.

Publikationer 1997 2018

Cumulative disadvantage: Reply to "Consider choices in the Gender in Science debate"

Dalgaard, L. T., 1 maj 2018, I : Acta Physiologica (Print). 223, 1, 3 s., e13052

Publikation: Bidrag til tidsskriftKommentar/debatForskningpeer review

Fil

Expression of miR-206 in human islets and its role in glucokinase regulation

Joglekar, M., Wong, W. W. K., Maynard, C. L., Umrani, M. R., Martin, D., Loudovaris, T., Thomas, H. E., Dalgaard, L. T. & Hardikar, A. A., 2018, I : American Journal of Physiology: Endocrinology and Metabolism. 315, 4, s. 634-637 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells

Geiger, J. & Dalgaard, L. T., 31 maj 2018, Mitochondrial Bioenergetics: Methods and Protocols. New York: Springer Nature, Bind 1782, s. 337-350 14 s. (Methods in Molecular Biology).

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningpeer review

Localization of microRNA-375 in perinatal rat pancreas.

Larsen, L., Rosenstierne, M. W., Dalgaard, L. T. & Nielsen, J. H., 2018, I : Non-Coding RNAs in Endocrinology. 2018, 3, 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Åben adgang

Mange fordomme små

Dalgaard, L. T., 27 jul. 2018, I : Weekendavisen.

Publikation: Bidrag til tidsskriftBidrag til avis - AvisartikelFormidling

Åben adgang

Projekter 2010 2017

Valgte aktiviteter 2007 2016

Roskilde Universitet (Organisation)

Dalgaard, L. T. (Medlem)
2014 → …

Aktivitet: MedlemsskabMedlemskab af udvalg, råd og nævn

Invited speaker

Dalgaard, L. T. (Foredragsholder)
2014

Aktivitet: Foredrag og mundtlige bidrag

Danish Diabetes Academy, Education Committee (Ekstern organisation)

Dalgaard, L. T. (Medlem)
2014 → …

Aktivitet: MedlemsskabMedlemskab af udvalg, råd og nævn

Invited speaker

Dalgaard, L. T. (Foredragsholder)
2014

Aktivitet: Foredrag og mundtlige bidrag

Invited speaker: Mitochondrial workshop

Dalgaard, L. T. (Foredragsholder)
2014

Aktivitet: Foredrag og mundtlige bidrag

Priser

Poul and Erna Sehested-Hansen Foundation Honor Bursary

Louise Torp Dalgaard (Modtager), 2013

Pris: Priser, stipendier, udnævnelser