Copepods swimming, respiration and stressrelated genes in response to high stocking densities

Birgitte Nilsson (Oplægsholder)

    Aktivitet: Tale eller præsentationForedrag og mundtlige bidrag


    B. Nilsson*, G. Drillet, H.H. Jakobsen, M. Rais, F. Lombard, E. Petkeviciute, S. Gasparini, B.W. Hansen.
    The utilization of calanoid copepods in aquaculture hatcheries faces a number of challenges. One major difficulty is rearing dense cultures, suggesting high density conditions may have a negative effect on cultivated copepod species. The conditions during high densities can result in different types of stressors. These include reduction of food resources, oxygen depletion, accumulation of metabolic products and the physical interactions with other individuals. Besides the production of resting eggs in response to crowding, the copepods might experience increased cannibalism of eggs and nauplii. When responding to stress, organisms usually increase their energy demand, which leads to an increased respiration. However, data obtained from literature do not show an effect on respiration by increased copepod densities. Based on previous studies, we expect that high density conditions do have a negative effect on calanoid copepods. In order to detect density induced stress in the calanoid copepod Acartia tonsa, we applied three physiological different end-points: swimming behavior, respiration, and a novel approach with the expression of stress related genes (heat shock protein 70, heat shock protein 90 and ferritin). We discuss results obtained by this three step recording strategy and evaluate detectability and feasibility of this stress toolbox.
    Periode23 aug. 201526 aug. 2015
    BegivenhedstitelAquaculture: cutting egde science in aquaculture
    Placeringmontpellier, Frankrig